S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 30 No.2 (1989)
Effect of Trace Metals on In Vitro Crystallization of Calcium Oxalate in Agarose Gel Media
Agarose Gel에서 칼슘수산 결정형성에 대한 미량금속의 영향
- Park, Won Hee; Lee, Chongwook
- Issue Date
- Seoul J Med, Vol.30 No.2, pp. 119-125
- Trace metals are components of urinary stones, though minute and exist also
in normal urine. There have been many studies concerning the role of these materials in
crystallization. In this experiment we examined the role of trace metals and the effect of
interaction of trace metals and promotors in crystallization of calcium oxalate.
We used the agarose gel model with 3 layered structure and added trace metals in various
concentrations into the upper layer of oxalate solution. The seed crystals of monosodium
urate, brushite, and hydroxyapatite were used as the promotor and added to the middle
layer of agarose gel. The lower layer was the agar containing calcium ions. These test tubes
were incubated at 3TC and crystals formed in middle layer were extracted and dried on
previously determined time schedule. These crystals were analysed qualitatively and quantitatively.
The X-ray diffractogram of these extracts revealed only whewellite. Iron (III), aluminum(lIl),
copper(II), and zinc(ll) were proved to inhibit crystallization of calcium oxalate. And the
inhibitory activity was proportional to the concentration of these trace metals. These inhibitory
activities were about 40%, 40%, 25%, and 25%, respectively in 0.01 M of trace metals
(CaCI2: trace metal = 10: 1 molar ratio). Tin(ll) did not inhibit simple crystallization of calcium
oxalate, but significantly inhibited the promoting process by 3 seed crystals.