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Proliferative Activity of Pituitary Mammotrophs in Culture : a Morphological Study on DNA Synthesis Using In Vitro Bromodeoxyuridine Labeling Method
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- Authors
- Issue Date
- 1993-12
- Citation
- Seoul J Med, Vol.34 No.4, pp. 237-243
- Keywords
- Mummotroph proliferation ; Bromodeoxvuridine ; Rat ; Anterior pituitary cell culture ; Double inununohistochemistrv
- Abstract
- The present study was designed to investigate the proliferative activity of
mammotroph in the long-term monolayer cultures of male rat pituitaries by use of
bromodeoxyuridineflsrdl.ll-labeltnq technique combined with double immunohistochemical
staining. Rat anterior pituitary cells were exposed to 100 uM BrdU for 4 hrs at 4,
7, 12, 15, 20, 25 and 30 days in the primary cultures. After fixation with modified
Camoy's fixative, double immunohistochemical staining with anti-BrdU and antiprolactin
antibody was performed. It was shown that the ratio of BrdU-Iabeled
mammotrophs per 100 mammotrophs(BrdU Labeling Index) was 8.2% at 4 day and 8.
0% at 7 day in our cultures. Thereafter, it decreased until 30 days(1.7%). These results
demonstrated that the increase in the proportion of mammotrophs observed in our previous
monolayer cultures is caused, at least in part, by the cell division of mammortrophs.
- ISSN
- 0582-6802
- Language
- English
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