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An Improved Indirect Enzyme-Linked Immunosorbent Assay (ELISA) Suitable for Mass Screening of Anti-Sperm Antibodies : 항정자 항체의 검출을 위한 간접 ELISA법의 고안
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- Authors
- Issue Date
- 1989-12
- Citation
- Seoul J Med, Vol.30 No.4, pp. 263-270
- Keywords
- Antisperm antibodies (ASA) ; Indirect ELISA ; Vasectomy
- Abstract
- An improved, indirect enzyme-linked immunosorbent assay (ELISA), which
detects antisperm antibodies (ASA) in serum, which appears to be a sensitive and easy
quantitative procedure for mass screening that is not available with conventional biologic
assay systems. Using known positive human antisperm antisera, it was found that the ELISA
method was quite reproducible at a wide spectrum of titers. When comparing the ELISA
method and the gelatin agglutination (GAT) with the tray agglutination test (TAT) using
heterologous mouse anti-human sperm antisera, it was found that the ELISA method was
more sensitive than the GAT or TAT by more than a three fold dilution factor. The sera of 85
vasectomized subjects were studied with the ELISA, GAT and TAT to determine ASA. We
concluded that, in busy laboratories with heavy workloads, this improved ELISA is a very
sensitive, easily reproducible and efficient method of mass screening for sperm immunity.
- ISSN
- 0582-6802
- Language
- English
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