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Studies on The Mechanism of Reactive Oxygen Species Inactivation of Brain Microsomal Na+-K+-ATPase
유독성 산소 대사물에 의한 뇌조직 마이크로좀 Na+-K+-ATPase 활성억제 기전에 관한 연구

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Authors
Chung, Myung-Hee; Kim, Jung-Shik; Son, Young-Sook; Choi, Kil-Soo; Lim, Jung-Kyoo; Park, Chan-Woong
Issue Date
1985-03
Publisher
Seoul National University College of Medicine
Citation
Seoul J Med, Vol.26 No.1, pp. 22-32
Keywords
Na+ -K+ -ATPaseOxygen radicals and Lipid peroxidation
Abstract
Effects of xanthine/xanthine oxidase on the brain microsomal fraction were
studied with respect to its Na+ -K+ -ATPase activity, lipid peroxidation and SH-group oxidation,
The treatment of the membrane fraction with xanthine/xanthine oxidase resulted in the
inactivation of Na+ -K+ -ATPase and a proportionate loss of the membrane SH-groups with
sparing production of malondialdehyde. The observed amount of malondialdehyde was much
less than that by another oxidizing agents, Cu + + - HzOz exhibiting the comparable degree of
the ATPase inactivation, The inactivated ATPase by xanthine/xanthine oxidase was not recovered
with various phospholipids. Among the quenchers, only those which prevented the
inactivation of the ATPase recovered the loss of the membrane SH-groups, and the extent of
SH-group recovery was correlated to that of the ATPase activity. Furthermore, SH-group-containing
compounds (cysteine and glutathione) prevented the ATPase a.gainst the inactivation
when the membrane fraction was treated with xanthine/xanthine oxidase in the presence
of them. Serine showed no effect in this respect The results obtained suggest that reactive
oxygen species inactivate brain microsomal Na + - K+ -ATPase primarily by interacting with
SH-groups, which appear to be more susceptible than membrane phospholipid components
to oxidative attack by the reactive oxygen species,
ISSN
0582-6802
Language
English
URI
https://hdl.handle.net/10371/5665
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 26 No.1 (1985)
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