S-Space College of Medicine/School of Medicine (의과대학/대학원) Parasitology and Tropical Medicine (기생충학전공) Journal Papers (저널논문_기생충학전공)
Differential diagnosis of Taenia asiatica using multiplex PCR
- Jeon, Hyeong-Kyu; Chai, Jong-Yil; Kong, Yoon; Waikagul, Jitra; Insisiengmay, Bounnaloth; Rim, Han-Jong; Eom, Keeseon S
- Issue Date
- Exp Parasitol. 2009 Feb;121(2):151-6. Epub 2008 Nov 5.
- Animals; DNA Primers/chemistry; DNA, Helminth/*chemistry/isolation & purification; DNA, Mitochondrial/chemistry; Diagnosis, Differential; Feces/parasitology; Humans; Polymerase Chain Reaction/*methods; Sensitivity and Specificity; Species Specificity; Taenia/*classification/genetics; Taeniasis/*diagnosis/parasitology
- Taenia asiatica and T. saginata are frequently confused tapeworms due to their morphological similarities and sympatric distribution in Asian regions. To resolve this problem, a high-resolution multiplex PCR assay was developed to distinguish T. asiatica infections from infection with other human Taenia tapeworms. For molecular characterization, the species specificity of all materials used was confirmed by sequencing of the cox1 gene. Fifty-two samples were analyzed in this study, comprising 20 samples of T. asiatica genomic DNA from China, Korea, and the Philippines; 24 samples of T. saginata from Belgium, Chile, China, Ethiopia, France, Indonesia, Korea, Laos, the Philippines, Poland, Taiwan, Thailand, and Switzerland; and 10 samples of T. solium from Cape Verde, China, Honduras, and Korea. The diagnostic quality of the results obtained using PCR and species-specific primers designed from valine tRNA and NADH genes was equal to that based on the nucleotide sequencing of the cox1 gene. Using oligonucleotide primers Ta4978F, Ts5058F, Tso7421F, and Rev7915, the multiplex PCR assay was useful for the differentially diagnosing T. asiatica, T. saginata, and T. solium based on 706-, 629-, and 474-bp bands.
- 1090-2449 (Electronic)
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