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Opposing effects of protein kinase A and C on capacitative calcium entry into HL-60 promyelocytes.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Se-Young | - |
dc.contributor.author | Song, Sook-Keun | - |
dc.contributor.author | Kim, Kyong-Tai | - |
dc.date.accessioned | 2010-03-11T06:43:02Z | - |
dc.date.available | 2010-03-11T06:43:02Z | - |
dc.date.issued | 1998-09 | - |
dc.identifier.citation | Biochemical Pharmacology 56: 561-567, 1998. | en |
dc.identifier.issn | 0006-2952 | - |
dc.identifier.uri | https://hdl.handle.net/10371/60099 | - |
dc.description.abstract | Treatment of HL-60 cells with thapsigargin, a microsomal Ca2+/ATPase inhibitor, led to depletion of intracellular calcium stores followed by capacitative calcium entry. Stimulation of adenylyl cyclase with forskolin enhanced thapsigargin-induced Ca2+ influx. The forskolin effect was confirmed by enhanced fluorescence quenching induced by Mn2+ entry into fura-2 loaded cells. 1,9-Dideoxy-forskolin, an inactive analog of forskolin, did not affect capacitative calcium entry. On the other hand, phorbol 12-myristate 13-acetate (PMA), an activator of protein kinase C, inhibited thapsigargin-induced Ca2+ entry. Histamine and prostaglandin E2 (PGE2) elevated intracellular adenosine 3′:5′-cyclic monophosphate (cAMP) levels and enhanced the thapsigargin-induced capacitative calcium entry. Incubation with N-[2-(p-bromocynnamylamino)ethyl]-5-isoquinolinesulfonamide (H89), an inhibitor of protein kinase A (PKA), blocked the forskolin effect, and GF109203X, an inhibitor of protein kinase C (PKC), blocked the phorbol 12-myristate 13-acetate effect. The results suggest that protein kinase A regulates capacitative calcium entry positively, but that protein kinase C regulates Ca2+ influx negatively. Furthermore, after differentiation of HL-60 promyelocytes with dimethylsulfoxide to granulocytes, the inhibitory effect of phorbol 12-myristate 13-acetate became more pronounced, whereas the stimulatory effect of prostaglandin E2 did not change. This result suggests that the regulation of capacitative calcium entry by protein kinase C and protein kinase A develops differently during differentiation. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | protein kinase A | en |
dc.subject | protein kinase C | en |
dc.subject | capacitative calcium entry | en |
dc.subject | HL-60 cells | en |
dc.title | Opposing effects of protein kinase A and C on capacitative calcium entry into HL-60 promyelocytes. | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 최세영 | - |
dc.contributor.AlternativeAuthor | 송숙근 | - |
dc.contributor.AlternativeAuthor | 김경태 | - |
dc.identifier.doi | 10.1016/S0006-2952(97)00660-6 | - |
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