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Opposing effects of protein kinase A and C on capacitative calcium entry into HL-60 promyelocytes.

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dc.contributor.authorChoi, Se-Young-
dc.contributor.authorSong, Sook-Keun-
dc.contributor.authorKim, Kyong-Tai-
dc.date.accessioned2010-03-11T06:43:02Z-
dc.date.available2010-03-11T06:43:02Z-
dc.date.issued1998-09-
dc.identifier.citationBiochemical Pharmacology 56: 561-567, 1998.en
dc.identifier.issn0006-2952-
dc.identifier.urihttps://hdl.handle.net/10371/60099-
dc.description.abstractTreatment of HL-60 cells with thapsigargin, a microsomal Ca2+/ATPase inhibitor, led to depletion of intracellular calcium stores followed by capacitative calcium entry. Stimulation of adenylyl cyclase with forskolin enhanced thapsigargin-induced Ca2+ influx. The forskolin effect was confirmed by enhanced fluorescence quenching induced by Mn2+ entry into fura-2 loaded cells. 1,9-Dideoxy-forskolin, an inactive analog of forskolin, did not affect capacitative calcium entry. On the other hand, phorbol 12-myristate 13-acetate (PMA), an activator of protein kinase C, inhibited thapsigargin-induced Ca2+ entry. Histamine and prostaglandin E2 (PGE2) elevated intracellular adenosine 3′:5′-cyclic monophosphate (cAMP) levels and enhanced the thapsigargin-induced capacitative calcium entry. Incubation with N-[2-(p-bromocynnamylamino)ethyl]-5-isoquinolinesulfonamide (H89), an inhibitor of protein kinase A (PKA), blocked the forskolin effect, and GF109203X, an inhibitor of protein kinase C (PKC), blocked the phorbol 12-myristate 13-acetate effect. The results suggest that protein kinase A regulates capacitative calcium entry positively, but that protein kinase C regulates Ca2+ influx negatively. Furthermore, after differentiation of HL-60 promyelocytes with dimethylsulfoxide to granulocytes, the inhibitory effect of phorbol 12-myristate 13-acetate became more pronounced, whereas the stimulatory effect of prostaglandin E2 did not change. This result suggests that the regulation of capacitative calcium entry by protein kinase C and protein kinase A develops differently during differentiation.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectprotein kinase Aen
dc.subjectprotein kinase Cen
dc.subjectcapacitative calcium entryen
dc.subjectHL-60 cellsen
dc.titleOpposing effects of protein kinase A and C on capacitative calcium entry into HL-60 promyelocytes.en
dc.typeArticleen
dc.contributor.AlternativeAuthor최세영-
dc.contributor.AlternativeAuthor송숙근-
dc.contributor.AlternativeAuthor김경태-
dc.identifier.doi10.1016/S0006-2952(97)00660-6-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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