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Optimization of embryo density and the volume of culture medium for an improvement of mouse parthenogenetic embryo development

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Authors
Roh, Sangho; Choi, Young-Joo; Min, Byung-Moo
Issue Date
2005-09
Publisher
한국동물번식학회
Citation
REPRODUCTIVE & DEVELOPMENTAL BIOLOGY 29: 145-147.
Keywords
ParthenogenesisEmbryo densityCulture medium volumeB6E2 F₁mouse
Abstract
Autocrine or paracrine mediators released by the early embryo are implicated in the support of embryonic development. Their mechanisms and optimal embryo density in the medium, however, are uncertain. This study was conducted to establish the optimal embryo density and culture medium volume in mouse parthenogenetic embryo culture. In experiment 1, culture of parthenogenetirally activated oocytes at a concentration of 2~4 embryos/ uL significantly improved development to the blastoryst stage (72%≤) compared with culture at the lower (0.2~1 embryos㎕, 0~37.5%) and the higher (5~6 embryos㎕, 30~53%) concentration for 120 h when the oocytes were cultured in a 5㎕ drop under mineral oil In experiment 2, the embryos cultured at a concentration of 2~4 embryos㎕ in a 10㎕ drop (81.1%) showed significantly higher blastocyst rates than those in a 5㎕ drop (68.5%). This study optimizes in vitro culture condition by modifying embryo density and the volume of culture medium It may give appropriate level of autocrine and/or paracrine factors to enhance viability and subsequent normal development of mouse parthenogenetic embryos in vitro.
ISSN
1738-2432
Language
English
URI
https://hdl.handle.net/10371/60101
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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