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Dexamethasone inhibits the formation of multinucleated osteoclasts via down-regulation of beta3 integrin expression

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Authors
Kim, Yong Hee; Jun, Ji-Hae; Woo, Kyung Mi; Ryoo, Hyun-Mo; Kim, Gwan-Shik; Baek, Jeong-Hwa
Issue Date
2006-08
Publisher
Pharmaceutical Society of Korea
Citation
Arch Pharm Res., 29, 691-698
Keywords
DexamethasoneOsteoclastVitronectin receptorβ3 IntegrinFusion
Abstract
Although glucocorticoids are known to affect osteoclast differentiation and function, there have been conflicting reports about the effect of glucocorticoids on osteoclast formation, leading to the assumption that microenvironment and cell type influence their action. We explored the effect of the synthetic glucocorticoid analog dexamethasone on the formation of osteoclasts. Dexamethasone inhibited the formation of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated osteoclasts without affecting the formation of TRAP-positive mononuclear cells in a coculture of mouse osteoblasts and bone marrow cells. Dexamethasone did not inhibit mRNA expression levels of the receptor activator of nuclear factor-kB ligand and osteoprotegerin, the essential regulators of osteoclastogenesis. Dexamethasone down-regulated the expression of β3 integrin mRNA and protein but did not alter expression of other osteoclast differentiation marker genes. Both dexamethasone and echistatin, a β3 integrin function blocker, inhibited TRAP-positive multinucleated osteoclast formation but not TRAP-positive mononuclear cell formation. These results suggest that dexamethasone inhibits the formation of multinucleated osteoclasts, at least in part, through the down-regulation of β3 integrin, which plays an important role in the formation of multinucleated osteoclasts.
ISSN
0253-6269
Language
English
URI
https://hdl.handle.net/10371/61890
DOI
https://doi.org/10.1007/BF02968254
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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