Publications
Detailed Information
Distinct sites in E-cadherin regulate different steps in Drosophila tracheal tube fusion.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Mihye | - |
dc.contributor.author | Lee, Seungbok | - |
dc.contributor.author | Zadeh, Alireza Dehghani | - |
dc.contributor.author | Kolodziej, Peter A. | - |
dc.date.accessioned | 2010-03-26T07:42:54Z | - |
dc.date.available | 2010-03-26T07:42:54Z | - |
dc.date.issued | 2003-12 | - |
dc.identifier.citation | Development 130(24), 5989-5999 | en |
dc.identifier.issn | 0950-1991 | - |
dc.identifier.uri | https://hdl.handle.net/10371/62040 | - |
dc.description.abstract | We have investigated how E-cadherin controls the
elaboration of adherens junction associated cytoskeletal structures crucial for assembling tubular networks. During Drosophila development, tracheal branches are joined at branch tips through lumens that traverse doughnut-shaped fusion cells. Fusion cells form E-cadherin contacts associated with a track that contains F-actin, microtubules, and Shot, a plakin that binds F-actin and microtubules. Live imaging reveals that fusion occurs as the fusion cell apical surfaces meet after invaginating along the track. Initial track assembly requires E-cadherin binding to b- catenin. Surprisingly, E-cadherin also controls track maturation via a juxtamembrane site in the cytoplasmic domain. Fusion cells expressing an E-cadherin mutant in this site form incomplete tracks that contain F-actin and Shot, but lack microtubules. These results indicate that Ecadherin controls track initiation and maturation using distinct, evolutionarily conserved signals to F-actin and microtubules, and employs Shot to promote adherens junction-associated cytoskeletal assembly. | en |
dc.description.sponsorship | The authors thank K. Miller, T. Uemura, M. Peifer and S. Myster,
A. Pacquelet, P. Rorth, S. Rogers, R. Vale, G. Beitel, U. Tepass and E. Giniger for providing fly stocks and reagents. Hanwei Cao provided excellent technical assistance. D. Greenstein,V. Bennett and anonymous reviewers provided helpful comments on the manuscript. One reviewer prompted us to carry out the co-expression experiment. We also thank A. Reynolds for helpful discussions. This research was supported by NIH RO1 GM62101 to P.A.K. | en |
dc.language.iso | en | en |
dc.publisher | Company of Biologists | en |
dc.subject | E-cadherin | en |
dc.subject | Drosophila | en |
dc.subject | Cytoskeleton | en |
dc.title | Distinct sites in E-cadherin regulate different steps in Drosophila tracheal tube fusion. | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이민혜 | - |
dc.contributor.AlternativeAuthor | 이민혜 | - |
dc.contributor.AlternativeAuthor | 이승복 | - |
dc.contributor.AlternativeAuthor | 이승복 | - |
dc.identifier.doi | 10.1242/dev.00806 | - |
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.