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P2X7 Receptor-mediated Membrane Blebbing in Salivary Epithelial Cells

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Authors
Park, Kyungpyo; Hwang, Sung-Min; Koo, Na-Youn; Choi, Se-Young; Chun, Gae-Sig; Kim, Joong-Soo
Issue Date
2009-06
Publisher
The Korean Physiological Society & the Korean
Citation
Korean J Physiol Pharmacol 13: 175-179
Keywords
P2X7 receptorMembrane blebbingROCKICa2+ influx
Abstract
High concentrations of ATP induce membrane blebbing. However, the underlying mechanism involved in epithelial cells remains unclear. In this study, we investigated the role of the P2X7 receptor (P2X7R) in membrane blebbing using Par C5 cells. We stimulated the cells with 5 mM of ATP for 1∼2 hrs and found the characteristics of membrane blebbing, a hallmark of apoptotic cell death. In addition, 500ՌM Bz-ATP, a specific P2X7R agonist, induced membrane blebbing. However, 300ՌM of Ox-ATP, a P2X7R antagonist, inhibited ATP-induced membrane blebbing, suggesting that ATP- induced membrane blebbing is mediated by P2X7R. We found that ATP-induced membrane blebbing was mediated by ROCK I activation and MLC phosphorylation, but not by caspase-3. Five mM of ATP evoked a biphasic [Ca2+]i response; a transient [Ca2+]i peak and sustained [Ca2+]i increase secondary to ATP-stimulated Ca2+ influx. These results suggest that P2X7R plays a role in membrane blebbing of the salivary gland epithelial cells.
ISSN
1226-4512
Language
English
URI
https://hdl.handle.net/10371/62079
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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