S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
p44/42 MAPK is necessary for receptor activator of nuclear factor-kB ligand induction by extracellular high calcium
- Kim, Gwan-Shik; Kim, Yong Hee; Kim, Jin Mi; Kim, Si Nae; Baek, Jeong-Hwa
- Issue Date
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 304 (2003) 729-735.
- Although extracellular calcium (Ca2+o) has been suggested to modulate bone remodeling, the exact mechanism is unclear. This study was performed to explore the signaling pathways of high Ca2+o that are responsible for controlling the expression of receptor activator of NF-κB ligand (RANKL) in mouse osteoblastic cells. As previously reported, high Ca2+o increased RANKL expression. However, the G protein-coupled Ca2+o-sensing receptor (CaSR) was not detected in the primary cultured mouse osteoblastic cell. The inhibition of the pertussis-sensitive G protein, phospholipase C, protein kinase C, intracellular calcium mobilization, p38 MAPK, or phosphoinositide 3-kinase did not block RANKL induction caused by high Ca2+o. In contrast, the inhibition of p44/42 MAPK pathway reduced the RANKL expression induced by high Ca2+o. Moreover, high Ca2+o activated p44/42 MAPK and MEK1/2. These results suggest that RANKL induction by high Ca2+o might not be mediated by CaSR and its putative downstream signaling pathways, but the pathway employing p44/42 MAPK is involved in the high Ca2+o-induced RANKL expression in mouse osteoblastic cells.
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