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DNA methyltransferase 3B mutant in ICF syndrome interacts non-covalently with SUMO-1

Cited 8 time in Web of Science Cited 9 time in Scopus
Authors
Park, Jinah; Kim, Tae-You; Jung, Yeonjoo; Song, Sang-Hyun; Kim, Sung-Hak; Oh, Do-Youn; Im, Seock-Ah; Bang, Yung-Jue
Issue Date
2008-09-03
Publisher
Springer Verlag
Citation
J Mol Med. 2008 Nov;86(11):1269-77.
Keywords
Cell Line, TumorCell Nucleus/metabolismCentromere/genetics*Chromosomal InstabilityCraniofacial Abnormalities/*geneticsDNA (Cytosine-5-)-Methyltransferase/genetics/*metabolismFaceHumansImmunologic Deficiency Syndromes/*geneticsInterleukin-8/geneticsNF-kappa B/metabolismPoint MutationPromoter Regions, GeneticProtein BindingProtein Inhibitors of Activated STAT/metabolismSUMO-1 Protein/*metabolismSmall Ubiquitin-Related Modifier Proteins/metabolismSyndromeTranscription Factors/metabolismTranscription, Genetic
Abstract
Mutations of the DNA methyltransferase 3B (DNMT3B) gene have been detected in patients with immunodeficiency, centromere instability, and facial anomalies (ICF) syndrome. Most of these mutations are clustered in its catalytic domain and thus lead to defective DNA methylation. Nevertheless, the S270P mutation in the N-terminal PWWP (Pro-Trp-Trp-Pro) domain of the DNMT3B gene has prompted questions as to how this mutation contributes to the development of ICF syndrome. In this study, we found that wild-type DNMT3B is SUMOylated through covalent modification, whereas the S270P mutant interacts with SUMO-1 via non-covalent interaction. The S270P mutation results in diffuse nucleus localization. Moreover, the S270P mutant fails to interact with PIAS1, a small ubiquitin-related modifier (SUMO) E3 ligase, and causes the constitutive activation of nuclear factor-kappa B, which induces the expression of interleukin 8. Collectively, our data demonstrate that the S270P mutation affects DNMT3B functions via specific, non-covalent interaction with SUMO-1.
ISSN
0946-2716 (Print)
Language
English
URI
http://hdl.handle.net/10371/62182
DOI
https://doi.org/10.1007/s00109-008-0392-5
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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