Publications
Detailed Information
Potentiation of PGE(2)-mediated cAMP production during neuronal differentiation of human neuroblastoma SK-N-BE(2)C cells.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Se-Young | - |
dc.contributor.author | Choi, Bo-Hwa | - |
dc.contributor.author | Suh, Byung-Chang | - |
dc.contributor.author | Chae, Hee-Don | - |
dc.contributor.author | Kim, Jong-So | - |
dc.contributor.author | Shin, Min-Jung | - |
dc.contributor.author | Kang, Shin-Sung | - |
dc.contributor.author | Negish, Manabu | - |
dc.contributor.author | Kim, Kyong-Tai | - |
dc.date.accessioned | 2010-03-31T05:15:14Z | - |
dc.date.available | 2010-03-31T05:15:14Z | - |
dc.date.issued | 2008-07 | - |
dc.identifier.citation | Journal of Neurochemistry. 2001, 79, 303-310. | en |
dc.identifier.issn | 0022-3042 | - |
dc.identifier.uri | https://hdl.handle.net/10371/62192 | - |
dc.description.abstract | The prostaglandin-evoked cAMP production was studied in human neuroblastoma SK-N-BE(2)C cells during neuronal differentiation induced by all-trans retinoic acid. The incubation with 5 µm all-trans retinoic acid for 4–6 days promoted neurite outgrowth of cells. After differentiation, prostaglandin E2 (PGE2)-induced cAMP production was dramatically increased, whereas forskolin- and AlF -induced cAMP productions were not changed. The increase reached maximum after 4-days of incubation with all-trans retinoic acid. The differentiation caused an increase in the maximal response and a decrease in the half-maximal effective concentration of the PGE2-induced cAMP production. In addition, the binding of [3H]PGE2 to membrane receptors was enhanced in differentiated cells. However, the order of potency of the various prostaglandins (PGE1 = PGE2 > PGD2 = PGF2α = PGI2) in cAMP production did not change during the differentiation, suggesting that mainly E-prostanoid (EP) receptors were involved. Butaprost, an EP2 receptor specific agonist, increased the cAMP level in a concentration dependent manner and had a similar potentiating effect on cAMP production as PGE2 upon differentiation. Northern blot analysis using the human cDNA probes shows that the EP2 mRNA level was about seven times higher in differentiated cells, while the dopamine β-hydroxylase (DBH) mRNA completely disappeared. Our results, thus, suggest that elevated gene expression of the prostanoid EP2 receptor results in an increase in the PGE2-evoked cAMP production in SK-N-BE(2)C cells during neuronal differentiation. | en |
dc.language.iso | en | en |
dc.publisher | International Society for Neurochemistry | en |
dc.subject | cAMP | en |
dc.subject | EP receptor | en |
dc.subject | neuroblastoma cells | en |
dc.subject | neuronal differentiation | en |
dc.subject | prostaglandin | en |
dc.subject | transcriptional regulation | en |
dc.title | Potentiation of PGE(2)-mediated cAMP production during neuronal differentiation of human neuroblastoma SK-N-BE(2)C cells. | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 최세영 | - |
dc.contributor.AlternativeAuthor | 최보화 | - |
dc.contributor.AlternativeAuthor | 서병창 | - |
dc.contributor.AlternativeAuthor | 채희돈 | - |
dc.contributor.AlternativeAuthor | 김종수 | - |
dc.contributor.AlternativeAuthor | 신민정 | - |
dc.contributor.AlternativeAuthor | 강신성 | - |
dc.contributor.AlternativeAuthor | 김경태 | - |
dc.identifier.doi | 10.1111/j.1471-4159.2001.00577.x | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.