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Effect of tumor necrosis factor-alpha on intracellular Staphylococcus aureus in vascular endothelial cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Wan Beom | - |
dc.contributor.author | Lee, Jae Hoon | - |
dc.contributor.author | Jeon, Jae Hyun | - |
dc.contributor.author | Lee, Su Jin | - |
dc.contributor.author | Kim, Sung Han | - |
dc.contributor.author | Kim, Nam Joong | - |
dc.contributor.author | Kim, Hong Bin | - |
dc.contributor.author | Oh, Myoung-Don | - |
dc.contributor.author | Choe, Kang Won | - |
dc.date.accessioned | 2010-03-31T05:42:07Z | - |
dc.date.available | 2010-03-31T05:42:07Z | - |
dc.date.issued | 2008-01-08 | - |
dc.identifier.citation | FEMS Immunol Med Microbiol. 2008 ;52(2):247-52. | en |
dc.identifier.issn | 0928-8244 (Print) | - |
dc.identifier.uri | https://hdl.handle.net/10371/62203 | - |
dc.description.abstract | Although tumor necrosis factor-alpha (TNF-alpha) is an important host factor against intracellular bacteria, little is known about the effect of TNF-alpha on the persistence of intracellular Staphylococcus aureus in vascular endothelial cells. It was investigated whether recombinant human TNF-alpha influences the survival of intracellular S. aureus (ATCC 29213) in human umbilical vein endothelial cells (HUVEC) under a condition with an antistaphylococcal agent, and its mechanism. The HUVECs were incubated with TNF-alpha, oxacillin, or both in 24-well plates for up to 48 h following internalization of S. aureus (10(6) CFU well(-1)) into HUVECs for 1 h. TNF-alpha (1 ng mL(-1)) significantly reduced the number of intracellular S. aureus in HUVECs, and TNF-alpha plus oxacillin eliminated more intracellular S. aureus in HUVEC than oxacillin alone. The LDH viability assay and quantification of apoptosis using photometric enzyme-immunoassay showed that TNF-alpha preferentially induced cell death and apoptosis of HUVECs infected with S. aureus compared with noninfected HUVECs. These results indicate that TNF-alpha helps antistaphylococcal antibiotics to eliminate intracellular S. aureus in vascular endothelial cells, partly because TNF-alpha preferentially induces apoptosis of endothelial cells infected by S. aureus. | en |
dc.language.iso | en | en |
dc.publisher | Federation of European Microbiological Societies | en |
dc.subject | Anti-Bacterial Agents/pharmacology | en |
dc.subject | Apoptosis | en |
dc.subject | Cell Line | en |
dc.subject | Colony Count, Microbial | en |
dc.subject | Endothelial Cells/*microbiology | en |
dc.subject | Humans | en |
dc.subject | L-Lactate Dehydrogenase/metabolism | en |
dc.subject | Microbial Viability | en |
dc.subject | Oxacillin/pharmacology | en |
dc.subject | Staphylococcus aureus/*drug effects | en |
dc.subject | Tumor Necrosis Factor-alpha/*immunology | en |
dc.title | Effect of tumor necrosis factor-alpha on intracellular Staphylococcus aureus in vascular endothelial cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 박완범 | - |
dc.contributor.AlternativeAuthor | 이재훈 | - |
dc.contributor.AlternativeAuthor | 전재현 | - |
dc.contributor.AlternativeAuthor | 이수진 | - |
dc.contributor.AlternativeAuthor | 김성한 | - |
dc.contributor.AlternativeAuthor | 김남중 | - |
dc.contributor.AlternativeAuthor | 김홍빈 | - |
dc.contributor.AlternativeAuthor | 오명돈 | - |
dc.contributor.AlternativeAuthor | 최강원 | - |
dc.identifier.doi | 10.1111/j.1574-695X.2007.00365.x | - |
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