Browse
S-Space
College of Dentistry/School of Dentistry (치과대학/치의학대학원)
Dept. of Dentistry (치의학과)
Journal Papers (저널논문_치의학과)
Expression of a sodium bicarbonate cotransporter in human parotid salivary glands
- Authors
- Issue Date
- 2002-01
- Publisher
- Pergamon
- Citation
- Archives of Oral Biology 47, (2002), 1-9
- Keywords
- bicarbonate secretion ; intracellular ph ; NBC
- Abstract
- The human parotid gland secretes much of the bicarbonate that enters the mouth. Prompted by studies of animal models, this study sought evidence for the expression of a functional Na+–HCO3− cotransporter (NBC) in human parotid acinar cells. Microfluorometric measurements of intracellular pH in isolated acini showed that the recovery from an acid load was achieved in part by HCO3− uptake via a Na+-dependent, DIDS-sensitive mechanism. By reverse transcriptase–polymerase chain reaction, a full-length NBC1 clone was obtained showing more than 99% homology with the human pancreatic isoform hpNBC1. Expressed in Xenopus oocytes, the electrogenicity of the transporter was detected as an inwardly directed, Na+- and HCO3−-dependent flux of negative charge. Immunohistochemistry using antibodies raised to NBC1 showed strong staining of the basolateral membrane of the acinar cells. Therefore, it was concluded that a functional electrogenic Na+–HCO3− cotransporter is expressed in the human parotid gland, and that it contributes to pH regulation in the acinar cells and could play a significant part in salivary secretion.
- ISSN
- 0003-9969
- Language
- English
- Files in This Item: There are no files associated with this item.
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.