S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Expression of a sodium bicarbonate cotransporter in human parotid salivary glands
- Park, K.; Hurley, P.T.; Roussa, E.; Cooper, G.J.; Smith, C.P.; Thevenod, F.; Steward, M.C.; Case, R.M.
- Issue Date
- Archives of Oral Biology 47, (2002), 1-9
- The human parotid gland secretes much of the bicarbonate that enters the mouth. Prompted by studies of animal models, this study sought evidence for the expression of a functional Na+–HCO3− cotransporter (NBC) in human parotid acinar cells. Microfluorometric measurements of intracellular pH in isolated acini showed that the recovery from an acid load was achieved in part by HCO3− uptake via a Na+-dependent, DIDS-sensitive mechanism. By reverse transcriptase–polymerase chain reaction, a full-length NBC1 clone was obtained showing more than 99% homology with the human pancreatic isoform hpNBC1. Expressed in Xenopus oocytes, the electrogenicity of the transporter was detected as an inwardly directed, Na+- and HCO3−-dependent flux of negative charge. Immunohistochemistry using antibodies raised to NBC1 showed strong staining of the basolateral membrane of the acinar cells. Therefore, it was concluded that a functional electrogenic Na+–HCO3− cotransporter is expressed in the human parotid gland, and that it contributes to pH regulation in the acinar cells and could play a significant part in salivary secretion.
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