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Bone-related gene profiles in developing calvaria
Cited 17 time in
Web of Science
Cited 18 time in Scopus
- Authors
- Issue Date
- 2006-05
- Publisher
- Elsevier
- Citation
- Gene 372 (2006) 71–81
- Keywords
- Bone ; Calvaria ; Osteogenesis ; Mircroarray ; Gene profile
- Abstract
- Generating a comprehensive understanding of osteogenesis-related gene profiles is very important in the development of new treatments for
osteopenic conditions. Developing calvaria undergoes a typical intramembranous bone-forming process. To identify genes associated with
osteoblast differentiation, we isolated total RNAs from parietal bones, that represent active osteoblasts, and sutural mesenchyme, that represents
osteoprogenitor cells, and comprehensively analyzed their gene expression profiles using an oligo-based Affymetrix microarray chip containing
22,690 probes. About 2100 genes with Present calls had more than 2-fold higher expression in bone compared to sutures while 73 of these genes
had more than 8-fold expression. Some of these genes are already known to be bone-related biomarkers: VitD receptor, bone sialoprotein,
osteocalcin, osteopontin, MMP13, etc. Eight genes were selected and subjected to confirmation by quantitative real-time RT-PCR analyses. All the
genes tested showed higher expression in bones, ranging from 5- to 140-fold. Several of these genes are ESTs while others are already known but
their functions in osteogenesis were not previously known. Most genes of the BMP and FGF families probed in the Genchip analysis were more
highly expressed in bone tissues compared to suture. All differentially-expressed Runx and Dlx family genes also showed higher expression in
bone. These results imply that our data is valid and can be used as a good standard for the mining of osteogenesis-related genes.
© 2005 Elsevier B.V. All rights reserved.
- ISSN
- 0378-1119
- Language
- English
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