S-Space College of Medicine/School of Medicine (의과대학/대학원) Parasitology and Tropical Medicine (기생충학전공) Journal Papers (저널논문_기생충학전공)
Proliferative effects of excretory/secretory products from Clonorchis sinensis on the human epithelial cell line HEK293 via regulation of the transcription factor E2F1
- Kim, Young Ju; Choi, Min-Ho; Hong, Sung-Tae; Bae, Young Mee
- Issue Date
- Springer Verlag
- Parasitol Res. 2008 Feb;102(3):411-7. Epub 2007 Nov 20.
- Animals; Cell Line; Clonorchiasis; Clonorchis sinensis/*physiology; E2F1 Transcription Factor/*genetics; Epithelial Cells/*parasitology/*physiology; Fish Diseases/parasitology; Fishes/parasitology; Gene Expression Regulation; Genes, Reporter; Humans; Kidney; Luciferases/genetics; RNA, Small Interfering/genetics; Transfection
- Clonorchis sinensis is one of the most prevalent parasitic helminths of humans in East Asia. Although several complications in bile duct epithelial cells are caused by C. sinensis infection, the mechanism is not clearly understood. To clarify the effects of C. sinensis excretory-secretory products (ES products) on bile duct epithelial cells, we investigated their effects on the human embryonic kidney epithelial cell line HEK293 in vitro. Our results show that ES products alter the proportion of cells in each stage of the cell cycle and induce HEK293 cell proliferation. Among cell cycle-related proteins, the expression of cyclin E increased markedly after treatment with ES products, indicating that the G1/S transition occurred. In addition, the expression of the transcription factor E2F1 was up-regulated by the addition of ES products. Small interfering RNA (siRNA) was used to demonstrate that the transcription factor E2F1 is a key factor in the control of cell proliferation in HEK293 cells. The present results demonstrate that ES products from C. sinensis stimulate cell proliferation by inducing E2F1 expression. We suggest that the ES products released from C. sinensis during infection may play an important role in the development of cholangiocarcinoma via the overgrowth of the bile duct epithelium.
- 0932-0113 (Print)
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