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Upregulated HSP27 in human breast cancer cells reduces Herceptin susceptibility by increasing Her2 protein stability

Cited 102 time in Web of Science Cited 94 time in Scopus
Authors
Kang, Se Hun; Kang, Keon Wook; Kim, Kyung-Hee; Kwon, Bumi; Kim, Seok-Ki; Lee, Ho-Young; Kong, Sun-Young; Lee, Eun Sook; Jang, Sang-Geun; Yoo, Byong Chul
Issue Date
2008-10-07
Publisher
BioMed Central
Citation
BMC Cancer 8:286-295
Keywords
Antibodies, Monoclonal/*pharmacologyAntineoplastic Agents/pharmacologyBreast Neoplasms/*drug therapy/genetics/*metabolism/pathologyCell Line, TumorDrug Resistance, NeoplasmEnzyme StabilityHSP27 Heat-Shock ProteinsHeat-Shock Proteins/*biosynthesis/geneticsHumansNeoplasm MetastasisNeoplasm Proteins/*biosynthesis/geneticsReceptor, erbB-2/*metabolismUp-Regulation
Abstract
BACKGROUND: Elucidating the molecular mechanisms by which tumors become resistant to Herceptin is critical for the treatment of Her2-overexpressed metastatic breast cancer. METHODS: To further understand Herceptin resistance mechanisms at the molecular level, we used comparative proteome approaches to analyze two human breast cancer cell lines; Her2-positive SK-BR-3 cells and its Herceptin-resistant SK-BR-3 (SK-BR-3 HR) cells. RESULTS: Heat-shock protein 27 (HSP27) expression was shown to be upregulated in SK-BR-3 HR cells. Suppression of HSP27 by specific siRNA transfection increased the susceptibility of SK-BR-3 HR cells to Herceptin. In the presence of Herceptin, Her2 was downregulated in both cell lines. However, Her2 expression was reduced by a greater amount in SK-BR-3 parent cells than in SK-BR-3 HR cells. Interestingly, co-immunoprecipitation analysis showed that HSP27 can bind to Her2. In the absence of Herceptin, HSP27 expression is suppressed and Her2 expression is reduced, indicating that downregulation of Her2 by Herceptin can be obstructed by the formation of a Her2-HSP27 complex. CONCLUSION: Our present study demonstrates that upregulated HSP27 in human breast cancer cells can reduce Herceptin susceptibility by increasing Her2 protein stability.
ISSN
1471-2407 (Electronic)
Language
English
URI
https://hdl.handle.net/10371/62516
DOI
https://doi.org/10.1186/1471-2407-8-286
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College of Medicine/School of Medicine (의과대학/대학원)Nuclear Medicine (핵의학전공)Journal Papers (저널논문_핵의학전공)
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