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Lipopolysaccharide from Prevotella nigrescens stimulates osteoclastogenesis in cocultures of bone marrow mononuclear cells and primary osteoblasts.

Cited 31 time in Web of Science Cited 31 time in Scopus
Authors
Lee, S.-B.; Chung, E.-J.; Kim, S.-J.; Kim, H.-H.; Kim, H.-M.; Ko, J. S.
Issue Date
2006-05
Publisher
Wiley-Blackwell
Citation
JOURNAL OF PERIODONTAL RESEARCH, 2006;41:288-296.
Keywords
cytokineslipopolysaccharideosteoclastogenesisPrevotella nigrescens
Abstract
Background and Objective: Lipopolysaccharide is thought to be a major virulence factor of pathogens associated with periodontal diseases and is believed to stimulate bone resorption in vivo. Although Prevotella nigrescens has been implicated in periodontitis, its role in osteoclastogenesis has not been reported. In this study, we investigated the effects of lipopolysaccharide from P. nigrescens on the formation of osteoclasts and the production of cytokines related to osteoclast differentiation.

Material and Methods: Mouse bone marrow mononuclear cells were cultured in the presence of macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor κB ligand (RANKL), with or without lipopolysaccharide. Bone marrow mononuclear cells were also cocultured with calvarial osteoblastic cells in the presence or absence of lipopolysaccharide. Osteoclast formation was determined by tartrate-resistant acid phosphatase cytochemistry. The production of osteoprotegerin (OPG), M-CSF, tumor necrosis factor alpha (TNF-α), transforming growth factor-beta (TGF-β) and prostaglandin E2 (PGE2) was determined by enzyme-linked immunosorbent assay (ELISA).

Results: P. nigrescens lipopolysaccharide inhibited osteoclast differentiation from bone marrow mononuclear cells cultured in the presence of M-CSF and RANKL. However, in the coculture system, P. nigrescens lipopolysaccharide stimulated osteoclastogenesis. Notably, P. nigrescens lipopolysaccharide decreased OPG production but increased TGF-β secretion. In addition, treatment with P. nigrescens lipopolysaccharide increased PGE2 production during the late stage of the culture period. There was no difference in M-CSF and TNF-α production.

Conclusion: These results demonstrate that P. nigrescens lipopolysaccharide stimulates osteoclastogenesis in the coculture system by decreasing the production of OPG and increasing the production of TGF-β and PGE2. Through the mechanisms involving these factors, P. nigrescens lipopolysaccharide may cause alveolar bone resorption in periodontal diseases.
ISSN
0022-3484
Language
English
URI
https://hdl.handle.net/10371/62711
DOI
https://doi.org/10.1111/j.1600-0765.2006.00876.x
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Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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