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Measurement of bacterial (E. coli) concentration by flow cytometry.

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Authors
Choi, Youngnim; Ji, Suk; Lee, Jung-ok
Issue Date
2005-06
Publisher
Korean Acadamy of Oral Biology
Citation
International Journal of Oral Biology. 30:65-69,2005.
Keywords
bacteriaconcentrationmeasurementflow cytometry abstract
Abstract
Periodontitis is a multi-microbial disease and the comparison of a series of periodomtopathogenic and nonperiodontopathogenic bacteria in terms of microbe-host interaction may provide clues to understand the microbial etiology of the disease better. When we deal with twenty different bacterial specles in a study, the first technical issue is how to measure the accurate concentration and use the same number of bacterial cells. We measured bacterial concentration by enumerating bacteria stained with SYTOX green for constant time using a flow c ytometer and compared the results with those obtained by plate counting. Concentrations claculated by two different methods were very close. Therefore, flow cytometric counting allowed the rapid analysis of liver/dead bacteria, offering the advantage of turbidity measurement and that of colony counting together.
ISSN
1226-7155
Language
English
URI
https://hdl.handle.net/10371/62780
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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