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Identification of mitochondrial F(1)F(0)-ATP synthase interacting with galectin-3 in colon cancer cells

Cited 10 time in Web of Science Cited 10 time in Scopus
Authors
Kim, Duck-Woo; Kim, Kyung Hee; Yoo, Byong Chul; Hong, Sung-Hye; Lim, Yong Chul; Shin, Young-Kyoung; Park, Jae-Gahb
Issue Date
2008-11-20
Publisher
Wiley-Blackwell
Citation
Cancer Sci. 2008 ;99(10):1884-91.
Keywords
Cell Cycle/physiologyCell Line, TumorColonic Neoplasms/*enzymology/*pathologyGalectin 3/*metabolismHumansIntracellular Membranes/metabolismMitochondria/*metabolismMitochondrial Proton-Translocating ATPases/*metabolismReproducibility of Results
Abstract
To evaluate the effect of galectin-3 in cell cycle regulation of colon cancer cells, we looked for binding molecules interacting with galectin-3 and examined the changes in cell cycle by suppressing galectin-3 and the binding molecule. To identify target molecules interacting with galectin-3, we analyzed immunoprecipitate of the anti-galectin-3 antibody obtained from human colon cancer cell line, using matrix-assisted laser desorption ionization-mass spectrometry. We validated subcellular localization of galectin-3 and ATP synthase identified, and ATP synthase activity was determined in the presence of galectin-3. Cell cycle regulation was monitored after galectin-3 siRNA transfection. ATP synthase b-subunit was identified in immunoprecipitate of the anti-galectin-3 antibody. Galectin-3 and ATP synthase were co-isolated in the inner membrane vesicles of mitochondria. Galectin-3 has an inhibitory activity against ATP synthase, and intracellular ATP content showed increasing tendency after galectin-3 suppression. Suppression of galectin-3 resulted in G0/G1 progression of human colon cancer cells arrested at S, S/G2 and G2/M phase in the presence of doxorubicin, and etoposide or nocodazole, respectively. Compared to cells in which ATP synthase d-subunit was suppressed alone, sub-G1 fraction caused by etoposide or nocodazole was decreased in cells with galectin-3 suppression alone. In conclusion, galectin-3 co-localized with ATP synthase in the inner membrane of mitochondria and has an inhibitory effect on ATP synthase in human colon cancer cells. In the presence of cell cycle synchronizing drugs, doxorubicin, etoposide, or nocodazole, suppression of galectin-3 induced cell cycle progression to G0/G1 phase.
ISSN
1349-7006 (Electronic)
1884-1891 (Print)
Language
English
URI
https://hdl.handle.net/10371/62805
DOI
https://doi.org/10.1111/j.1349-7006.2008.00901.x
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College of Medicine/School of Medicine (의과대학/대학원)Surgery (외과학전공)Journal Papers (저널논문_외과학전공)
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