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Microrough titanium surface affects biologic response in MG63 osteoblast-like cells

Cited 95 time in Web of Science Cited 98 time in Scopus
Authors
Lim, Young-Jun; Kim, Myung-Joo; Kim, Chang-Whe; Heo, Seong-Joo
Issue Date
2006-10
Publisher
Wiley-Blackwell
Citation
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 2006;79A:1023-1032.
Keywords
surface analysismicrorough titanium surfacecell numberalkaline phosphataseRunx2 gene expressiontype I collagen
Abstract
The purpose of this study was to define the surface properties of prepared titanium (Ti) disks, which served as a model system, and to contrast the biologic response of MG63 cells exposed to Ti disks with different levels of surface roughness. The surface properties interact with each other, resulting in a change of other surface qualities in addition to roughness due to the surface roughening procedure. The machined Ti disks were roughened by sandblasting and electric glow discharging. The surface properties of the Ti specimens were inspected through a comprehensive surface analysis. MG63 cell behaviors were compared along with cell number, alkaline phosphatase (ALP) activity, Runx2 gene expression, and type I collagen production. Statistics were evaluated, using analysis of variance (ANOVA). The sandblasted Ti disks demonstrated well-controlled surface roughness features and meaningful average roughness ranges, including the surface roughness of the modern microrough implant, used clinically. With increasing Ti surface roughness, the cell number decreased, while the ALP activity, type I collagen production, and Runx2 gene expression increased significantly. The rougher the Ti surface was, the sooner the Runx2 gene was expressed. Based on these results, we suggest that the microrough Ti surfaces of the 1-3 m range may contribute effectively to osteogenic differentiation and proliferation in MG63 cells. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006
ISSN
1549-3296
Language
English
URI
http://hdl.handle.net/10371/62863
DOI
https://doi.org/10.1002/jbm.a.31040
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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