토끼에 있어서 화상, 지혈대 결찰 및 수소이온 농도의 총혈액량과 조직 혈액량에 미치는 영향

Abstract

Alterations in total blood volume, plasma volume (T· 1824), extracellular fluid (NaSCN) and tissue blood volume (Cr 51--labeled red blood cell) were measured in 50 nembutalized rabbits. Animals were divided into 5 groups: the control group consisted of 14 rabbits. and in the second (8 rabbits) group after ten minutes of tourniquet release blood volumes were determined. The length of tourniquet application on both of hindlimbs was 30 minutes. In the third group of 12 rabbits burn was applied on 1/4 of the body surface area by means of immersing in the boiling water for 20 seconds. In the fourth group consisting of 10 rabbits acidosis was induced by means of injecting 0.1 N HCI intravenously. The fifth group was alkalosis group in which sodium bicarbonate was injected intravenously. The following results were obtained. 1. The normal plasma volume in rabbits was 4.86± 0.264% body weight. Total blood volume (T-1824 and hematocrit method) was 7.90±0.468%. and extracellular fluid volume was 25.2±0.839% body weight. 2. After ten minutes of tourniquet release there was no alteration in plasma and blood volume. Extracellular fluid volume. however. increased to 29.3%. In the burn group. plasma volume increased to 6.86% and blood volume increased to 11.34% accompanied by a slight tendency of increase in extracellular fluid volume. In the acidosis and alkalosis group extracellular fluid volume increased to 31.6%. but plasma and blood volume remained unaltered. 3. Tissue blood volums expressed pl/gm in the various tissues of normal rabbits were: brain 11.6. thyroid gland 116. heart 84.4. liver 95.7. lung 220. spleen 130. adrenal gland 58.8. 4. In the burn group tissue blood volumes of every tissue showed an increase. 5. In the tourniquet group heart and adrenal gland showed increases in the tissue blood volume. In the other tissues there was no significant alteration in the tissue blood volume. 6. Tissue blood volume of heart and lung increased when hydrogen ion concentration was changed. Spleen tissue blood volume increased in acidosis. Significant alteration in tissue blood volume was not observed in other tissues in spite of the change in hydrogen ion concentration.