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고온 및 한냉 환경이 흰쥐의 C14-포도당 대사에 미치는 영향 : Effects of Exposure to Warm and Cold Environments on Metabolism Of CILglueose in rats

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서울대학교 의과대학
Seoul J Med, Vol.6 No.2, pp. 21-28
The role of C14·glucose in energy metaboism of rats
were studied in twenty six rats under warm and cold
environment. Rats were kept in a constant temperature
animal chamber of which temperature was
maintained at 20°C in the first control group, the
second group animals were kept at 4°C and the third
warm acclimated group animals were kept at 33°C.
In every run of experiment, hourly blood and respiratory
C02 samples were collected after single injection
of C14-glucose. Bloods gas samples were analyzed for
blood sugar levels, total C02 production rates and their
On the basis of specific activities(SA)-time curves of
blood glucose and respiratory C02 in which both SA
decreased exponentially with time, glucose pools, glucose
spaces, relative specific activities, turnover rates of
glucose pool and relative glucose disappearance(RGD)
were calculated by isotope dilution method in each
The following data were obtained.
1. Average blood sugar level was 75±11 mg % in the
first group, 102±15mg % in the second group and
77±9. 0 mg % in the third group. The concentrations
of liver glycogen averaged 5.3±1.2 mg/gm in the
first group, 4.8±1.5 mg/gm in the second group and
7.3±2.3mg/gm in the third group, respectively.
In the cold acclimated group, blood sugar level
was elevated and concentration of liver glycogen
was decreased compared with the control group.
However, there were no remarkable differences
in the blood sugar level and concentration of liver
glycogen between control and warm acclimated
2. Average glucose pool size was 15.4±3.0 mg/lOO gm
in the first group, 28.6±2.0 mg/lOO gm in the second
group and 17.2±3.1mg/IOOgm in the third group.
Glucose space in each group was 20.9±6.9 % in the
first group and 28.0±5.0 % in second group and
22.4±3.1 % in the third group, respectively.
Values in glucose pool and glucose space were
remarkably increased in the cold acclimated group.
However there were little differences between warm
acclimated group compared with control group.
3. There were little differences in the turnover rates
of glucose pools between each group, showing 95.8±
19.0 %/hr in the first group, 98.0±8.7%/hr in the second group and 81.6±9.8 %/hr in the third group,
However, glucose consumption rate in the cold
acclimated group increased remarkably because of
their larger pool size of glucose.
4. The total CO2 production rate was a mean of
1.56±0.11 mM/hr/IOO gm in the first group, 1.96±
O. 2mM/hr/100 gm in the second group and 1.30±
0.15 mM/hr/lOO gm in the third group. Relative
specific activities(RSA) which represent the fraction
of CO2 derived from glucose to total CO2 production
rate averaged 13.I±3.0 % in the first group, 18.I±
1.2 % in the second group and 116±1.9 % in the
third group.
The CO2 delivery rate from glcose or oxidation
of glucose in the cold acclimated group was remarkably
increased because of increases in both values
of total CO2 production rate and RSA. On the other
hand, oxidation rate of glucose was decreased in
the warm acclimated group.
The relative glucose disappearances into CO2
(RGD) which are the fractions of glucose oxidized
into respiratory CO2 to total glucose consumption
rates were calculated mean of 41.5±11.7 % in the
control, 37.8±6.5 % in the cold acclimated group
and 32.8±4. 4 % in the warm acclimated group.
From the data of RGD in each group, rates of
glucose oxidation were proportionatly increased with
glucose disappearance rates.
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