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Comparison of the MicroScan, VITEK 2, and Crystal GP with 16S rRNA sequencing and MicroSeq 500 v2.0 analysis for coagulase-negative Staphylococci
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Miyoung | - |
dc.contributor.author | Heo, Se Ran | - |
dc.contributor.author | Choi, Soon Hee | - |
dc.contributor.author | Kwon, Hyelin | - |
dc.contributor.author | Park, Jeong Su | - |
dc.contributor.author | Seong, Moon-Woo | - |
dc.contributor.author | Lee, Do-Hoon | - |
dc.contributor.author | Park, Kyoung Un | - |
dc.contributor.author | Song, Junghan | - |
dc.contributor.author | Kim, Eui-Chong | - |
dc.date.accessioned | 2010-04-13T04:47:14Z | - |
dc.date.available | 2010-04-13T04:47:14Z | - |
dc.date.issued | 2008-12-25 | - |
dc.identifier.citation | BMC Microbiol. 2008 Dec 23;8:233. | en |
dc.identifier.issn | 1471-2180 (Electronic) | - |
dc.identifier.issn | 1471-2180 (Linking) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19105808 | - |
dc.identifier.uri | http://www.biomedcentral.com/content/pdf/1471-2180-8-233.pdf | - |
dc.identifier.uri | https://hdl.handle.net/10371/63095 | - |
dc.description.abstract | BACKGROUND: Three phenotypic identification systems (MicroScan, VITEK 2, and Crystal GP) were evaluated for their accuracy to identify coagulase-negative staphylococci (CNS). A total of 120 clinical isolates confirmed to be CNS via 16S rRNA sequencing and analysis with the MicroSeq 500 v2.0 database were assessed. RESULTS: The MicroScan, VITEK 2, and Crystal GP systems correctly identified 82.5%, 87.5%, and 67.5% of the isolates, respectively. Misidentification was the main problem in MicroScan (10.8%) and Crystal GP (23.3%) systems, whereas the main problem of VITEK 2 was low-level discrimination (7.5%). CONCLUSION: None of the 3 phenotypic systems tested could accurately and reliably identify CNS at the species level. Further verifications such as biochemical testing or 16S rRNA sequencing together with analysis using a comparable database might be helpful in this regard. | en |
dc.language.iso | en | en |
dc.publisher | BioMed Central | en |
dc.subject | Bacteriological Techniques/*methods/*standards | en |
dc.subject | Coagulase/metabolism | en |
dc.subject | Phenotype | en |
dc.subject | RNA, Ribosomal, 16S/genetics | en |
dc.subject | Reproducibility of Results | en |
dc.subject | Staphylococcal Infections/microbiology | en |
dc.subject | Staphylococcus/*classification/enzymology/genetics/*physiology | en |
dc.title | Comparison of the MicroScan, VITEK 2, and Crystal GP with 16S rRNA sequencing and MicroSeq 500 v2.0 analysis for coagulase-negative Staphylococci | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김미영 | - |
dc.contributor.AlternativeAuthor | 최순희 | - |
dc.contributor.AlternativeAuthor | 권혜린 | - |
dc.contributor.AlternativeAuthor | 박정수 | - |
dc.contributor.AlternativeAuthor | 성문우 | - |
dc.contributor.AlternativeAuthor | 이도훈 | - |
dc.contributor.AlternativeAuthor | 박경운 | - |
dc.contributor.AlternativeAuthor | 허세란 | - |
dc.contributor.AlternativeAuthor | 송정한 | - |
dc.contributor.AlternativeAuthor | 김의종 | - |
dc.identifier.doi | 10.1186/1471-2180-8-233 | - |
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