S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 29 No.4 (1988)
HBsAg Mediated Leukocyte Adherence Inhibition (LAI) and Its Immunologic Mechanism
HBsAg 항원 특이성 백혈구 접착억제 및 그 기전에 관한 연구
- Seo, Jeong Kee
- Issue Date
- Seoul J Med, Vol.29 No.4, pp. 361-371
- Leukocyte adherence Inhibition; Hepatitis B surface antigen-antibody system; Immunologic mechanism
- The leukocyte adherence inhibition (LAI) was studied in the hepatitis B viral
infection and its immunologic mechanism was assessed.
Leukocytes from 34 subjects with antiHBs in their serum (group A), leukocytes from 52
normal control subjects without any hepatitis B serologic markers (group B) and leukocytes
from 26 subjects with HBsAg in their serum (group C) were tested against HBsAg as a
specific antigen and BSA as a nonspecific antigen. The mean Non Adherence Index (NAI) of
group A was significantly higher than that of group B or of group C (P < 0.001). The NAIs
of 29 subjects (85.3%) among 34 subjects in group A were over 50 while only two subjects
(3.8%) in group B and only one subject (3.9%) in group C were over 50.
To determine whether soluble mediators such as leukocyte adherence inhibition factor
(LAIF) or immunoglobulin produced by sensitized Iymphcytes were involved in the LAI
response, supernatants collected from the peripheral blood leukocytes (PBL) after 24 hourincubation
at 37°C with HBsAg (200 ug/ml) were assessed for adherence inhibition activity
on normal PBL The supernatant collected following incubation of HBsAg with leukocytes
obtained from anti HBs (+), LAI (+) subjects markedly reduced the adherence of the
To determine the cell subfractions involved in the LAI assay, T and B cell enriched
subpopulations were tested for LAI reactivity to HBsAg. The T cell enriched subpopulation
gave positive LAI reactivity while the B cell enriched subpopulation gave no detectable
To investigate serum factors of immune donors, the LAI-nonreactive leukocytes from normal
control subjects or HBsAg carriers were preincubated with serum from LAI-reactive,
antiHBs (+) subjects or with hepatitis B immune globulin (HBIG), and were tested for LA!.
They showed positive LAI reactivity to HBsAg.
The present study showed specific HBsAg mediated LAI assay and suggested that both two
mechanisms, one dependent on specific antibody and the other on LAIF may be operated in
hepatitis B virus antigen -antibody system.