PCR Amplification of DNA Extracted from Paraffin-Embedded Tissue

Abstract

The polymerase chain reaction(PCR) DNA amplification method is a powerful new tool in the field of individual identification or examination of medicolegal evidences. Usually DNA is extracted from fresh tissue, blood, semen or dried specimen. We try to find suitable way to obtain DNA from paraffin-embedded tissue(PET), the most common preparation in pathological archives. Since PET processing conditions vary in their suitability for amplification, variable methods l)simple long incubation 2) xylene method 3)heating method 4)ether method were tried. Two VNTR loci of 017530 and apolipoprotein B gene were used for PCR amplification. The deparaffination process using ether shows the best result in the DNA extraction and PCR amplication.