S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 29 No.3 (1988)
Effect of Higenamine on ³H-Norepinephrine Release In the Rabbit Pulmonary Artery
토끼 폐동맥에서 Higenamine의 3H-Norepinephrine 유리에 대한 효과
- Wie, Bong-Ae; Park, Chan-Woong; Kim, Bong-Ki; Lim, Jung-Kyoo; Kim, Myung-suk; Myung, Ho-Jin
- Issue Date
- Seoul J Med, Vol.29 No.3, pp. 237-243
- It has been reported that higenamine increased the cardiac output and the
heart rate and decreased the systemic vascular resistance and blood pressure by stimulating
(3 -adrenoceptors. This study was undertaken to investigate the effects of higenamine on the
pre- and post-synaptic adrenergic neurotransmission in the spiral strips of pulmonary arteries
of New Zealand white rabbits. As the pre-synaptic response, the spontaneous and stimulationinduced
release of 3H-norepinephrine eH-NE), and as the post-synaptic response, the contractile
response were measured. The results were as follows:
1. Higenamine (3 X 10-6, 3 X 10-5 and 10-4M) increased both spontaneous and stimulation-
induced 3H-NE release dose-dependently, by 20-90% and 20-220%, respectively
but decreased the contractile response in a dose-dependent manner.
2. The effect of higenamine in increase of spontaneous and stimulation-induced 3H-NE
release was partially inhibited by the treatment of propranolol (3 X 10'--6M), but the effect
in contractile response was completely blocked. Isoproterenol (1.3 X 10-8
, 1.3 X 10- 7
and 1.3 X 10-6M) had no effect on spontaneous and stimulation-induced 3H-NE release
and contractile response.
3. The effect of higenamine was not inhibited by treatment with cocaine (3 X 10-5M).
4. The a z-adrenoceptor antagonist, yohimbine (3 X 10-8 and 3 X 10-7M) increased the
stimulation-induced 3H-NE release but did not affect spontaneous release.
5. Papaverine (3 X 10-6, 3 X 10-5 and 10-4M) increased spontaneous and stimualtioninduced
3H-NE release in a dose-dependent manner.
These results sugest that higenamine increases neurotransmitter release by the mechanism of
presynaptic action through the enhancement of cyclic AMP level in addition to stimulation of
the presynaptic (3 -receptor and that the mechanism of post-synaptic action of higenamine
lies in the stimulation of (3 -adrenoceptors.