A Study on Chemical Mediator of Human Mast Cells - Histamine Release from Human Lung and Tonsillar Mast Cells-

Abstract

Releasability of histamine, a granule-associated preformed chemical mediator, was observed from enzymatically dispersed human lung and tonsillar mast cells. IgE-dependent and calcium ionophore A23187 stimulated histamine release from enzymatically dispersed human lung mast cells was increased in dose-dependent fashion. Histamine release reached a maximum in the 1:30 dilution of anti-IgE and at 1.0 ,uM of calcium ionophore A23187 with human lung mast cells. Above the concentration of secretagogues which caused maximum histamine release, no further release occurred. Almost identical results were obtained with enzymatically dispersed human tonsillar mast cells. Increasing extracellular calcium concentration from 0.05 to 0.5mM caused a concentrationrelated increase in histamine release from enzymatically dispersed human lung mast cells.