Study on In vitro Fertilization of Mouse

Abstract

The success of human in vitro fertilization (IVF) and embryo transfer (ET) has focused attention on the culture conditions that can provide optimal development of the preimplantation embryo. Studies of in vitro fertilization using mouse have direct implications to human IVF, since similar conditions are used for both species. Mouse IVF was studied with special reference to the condition of culture media and vessels since April 1984. The results were summarized as follows: 1. Egg retrieval after superovulation in mice was 17.14±4.79 eggs per mouse. 2. About fifty percent of eggs retrieved showed good quality which has no evidence of fragmentation of the ooplasm or globules of unequal size. 3. In comparison with two media and two culture vessels after 72 hours in vitro culture, cleavage rates (six to eight-cell blastocyst) were 9.7% (Ham's F-10 in dish), 10.9% (Ham's F-10 in tube), 21.0% (m-KRB in dish), and 36.6% (m-KRB in tube) respectively. 4. Am-KRB medium was superior to Ham's F-10 in developing mouse embryo. It was suggested that mouse IVF system has a valuable place in a quality control system of human IVF and a research model.