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Cellular uptake of magnetic nanoparticle is mediated through energydependent endocytosis in A549 cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jun-Sung | - |
dc.contributor.author | Yoon, Tae-Jong | - |
dc.contributor.author | Yu, Kyeong Nam | - |
dc.contributor.author | Noh, Mi Suk | - |
dc.contributor.author | Woo, Minah | - |
dc.contributor.author | Kim, Byung-Geol | - |
dc.contributor.author | Lee, Kee-Ho | - |
dc.contributor.author | Sohn, Byung-Hyuk | - |
dc.contributor.author | Park, Seung-Bum | - |
dc.contributor.author | Lee, Jin-Kyu | - |
dc.contributor.author | Cho, Myung-Haing | - |
dc.date.accessioned | 2009-08-07T02:28:29Z | - |
dc.date.available | 2009-08-07T02:28:29Z | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | J. Vet. Sci 2006, 7, 321–326 | en |
dc.identifier.issn | 1229-845X | - |
dc.identifier.uri | http://www.vetsci.org/2006/abstract/321a.html | - |
dc.identifier.uri | https://hdl.handle.net/10371/6451 | - |
dc.description.abstract | Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye, rhodamine B isothiocyanate (RITC), within a silica shell [50 nm size, MNP@SiO2(RITC)s] were synthesized. For future application of the MNP@SiO2(RITC)s into diverse areas of research such as drug or gene delivery, bioimaging, and biosensors, detailed information of the cellular uptake process of the nanoparticles is essential. Thus, this study was performed to elucidate the precise mechanism by which the lung cancer cells uptake the magnetic nanoparticles. Lung cells were chosen for this study because inhalation is the most likely route of exposure and lung cancer cells were also found to uptake magnetic nanoparticles rapidly in preliminary experiments. The lung cells were pretreated with different metabolic inhibitors. Our results revealed that low temperature disturbed the uptake of magnetic nanoparticles into the cells. Metabolic inhibitors also prevented the delivery of the materials into cells. Use of TEM clearly demonstrated that uptake of the nanoparticles was mediated through endosomes. Taken together, our results demonstrate that magnetic nanoparticles can be internalized into the cells through an energy-dependent endosomal-lysosomal mechanism. | en |
dc.description.sponsorship | This work is supported by NANO Systems Institute-National
Core Research Center (NSI-NCRC), Korea Science and Engineering Foundation (KOSEF). Dr. Kee-Ho Lee is supported by grants from the Frontier Functional Human Genome Project and Nuclear National R & D Program of the Ministry of Science and Technology, Korea. The authors express deep thanks to Prof. Chanhee Chae, College of Veterinary Medicine, Seoul National University for his kind discussion of TEM and to Dr. Sang-Hyun Yun, Department of Materials Science and Engineering, Pohang University of Science and Technology, for assistance in the use of the TEM. | en |
dc.language.iso | en | - |
dc.publisher | 대한수의학회 = The Korean Society of Veterinary Science | en |
dc.subject | A549 cells | en |
dc.subject | cellular uptake | en |
dc.subject | endocytosis | en |
dc.subject | magnetic nanoparticle | en |
dc.title | Cellular uptake of magnetic nanoparticle is mediated through energydependent endocytosis in A549 cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김준성 | - |
dc.contributor.AlternativeAuthor | 윤태종 | - |
dc.contributor.AlternativeAuthor | 유경남 | - |
dc.contributor.AlternativeAuthor | 노미숙 | - |
dc.contributor.AlternativeAuthor | 우민아 | - |
dc.contributor.AlternativeAuthor | 김병걸 | - |
dc.contributor.AlternativeAuthor | 이기호 | - |
dc.contributor.AlternativeAuthor | 손병혁 | - |
dc.contributor.AlternativeAuthor | 박승범 | - |
dc.contributor.AlternativeAuthor | 이진규 | - |
dc.contributor.AlternativeAuthor | 조명행 | - |
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