Browse

Development of immunoassays for the detection of kanamycin in veterinary fields

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
Jin, Yong; Jang, Jin-Wook; Han, Chang-Hoon; Lee, Mun Han
Issue Date
2006
Publisher
대한수의학회 = The Korean Society of Veterinary Science
Citation
J. Vet. Sci 2006, 7, 111–117
Keywords
competitive direct ELISAimmunochromatographic assaykanamycinmonoclonal antibody
Abstract
Monoclonal antibody against kanamycin was prepared, and competitive direct ELISA and immunochromatographic assay were developed using the antibody to detect kanamycin in animal plasma and milk. The monoclonal antibody produced was identified to be IgG1, which has a kappa light chain. No cross-reactivity of the antibody was detected with other aminoglycosides, indicating that the monoclonal antibody was highly specific for kanamycin. Based on competitive direct ELISA, the detection limits of kanamycin were determined to be 1.1 ng/ml in PBS, 1.4 ng/ml in plasma, and 1.0 ng/ml in milk. The concentration of intramuscularly injected kanamycin was successfully monitored in rabbit plasma with competitive direct ELISA. Based on the colloidal gold-based immunochromatographic assay, the detection limits of kanamycin were estimated to be about 6-8 ng/ml in PBS, plasma, and milk. The immunochromatographic assay would be suitable for rapid and simple screening of kanamycin residues in veterinary medicine. Screened positives can be confirmed using a more sensitive laboratory method such as competitive direct ELISA. Therefore, the assays developed in this study could be used to complement each other as well as other laboratory findings. Moreover, instead of slaughtering the animals to obtain test samples, these methods could be applied to determine kanamycin concentration in the plasma of live animals.
ISSN
1229-845X
Language
English
URI
http://www.vetsci.org/2006/abstract/111a.html

http://hdl.handle.net/10371/6452
Files in This Item:
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse