S-Space College of Veterinary Medicine (수의과대학) Dept. of Veterinary Medicine (수의학과) Journal Papers (저널논문_수의학과)
In vivo induction of mucosal immune responses by intranasal administration of chitosan microspheres containing Bordetella bronchiseptica DNT
- Kang, Mi Lan; Kang, Sang Gyun; Jiang, Hu-Lin; Shin, Seung Won; Lee, Deog Yong; Ahn, Jeong-Min; Rayamahjia, Nabin; Park, In-Kyu; Shin, Sung Jae; Cho, Chong-Su; Yoo, Han Sang
- Issue Date
- Eur. J. Pharm. Biopharm. 63 (2006) 215–220
- Bordetella bronchiseptica; DNT; Chitosan microspheres; Mucosal immunity; Nasal administration
- In vitro immune-stimulating activities of Bordetella bronchiseptica dermonecrotoxin (BBD)-loaded in chitosan microspheres (CMs)
were reported with a mouse alveolar macrophage cell line (RAW264.7). Based on the report, in vivo activity of immune-induction
was investigated by intranasal administration of the BBD-loaded CMs into mice. BBD was loaded into the CMs prepared by an ionic
gelation process with tripolyphosphate. Mice were immunized by direct administration of the BBD-loaded CMs into the nasal cavity.
After immunization of the mice, BBD-specific immune responses (IgG and IgA titers) were measured in sera, nasal wash, and saliva
by ELISA. BBD-specific IgA titers in the nasal cavity were time- and dose-dependently increased by the administration. Similar phenomena
were observed in the analysis of systemic IgA and IgG in sera. However, the antibody in saliva was undetectable by ELISA. These
results suggested that direct vaccination via the nasal cavity was effective for targeting nasal-associated lymphoid tissues, and that CMs
were an efficient adjuvant in nasal mucosal immunity for atrophic rhinitis vaccine.
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