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DNA Barcoding Korean Birds

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Authors
Yoo, Hye Sook; Eah, Jae-Yong; Kim, Jong Soo; Kim, Young-Jun; Min, Mi-Sook; Paek, Woon Kee; Lee, Hang; Kim, Chang-Bae
Issue Date
2006
Publisher
한국분자·세포생물학회 = Korean Society of Molecular and Cellular Biology
Citation
Mol. Cells. 22, 323-327
Keywords
BirdDNA BarcodeKoreaMitochondrial Cytochrome c Oxidase IPhylogeny
Abstract
DNA barcoding, an inventory of DNA sequences from a
standardized genomic region, provides a bio-barcode
for identifying and discovering species. Several recent
studies suggest that the sequence diversity in a 648 bp
region of the mitochondrial gene for cytochrome c oxidase
I (COI) might serve as a DNA barcode for identifying
animal species such as North American birds, insects
and fishes. The present study tested the effectiveness
of a COI barcode in discriminating Korean bird
species. We determined the 5′ terminus of the COI barcode
for 92 species of Korean birds and found that species
identification was unambiguous; the genetic differences
between closely related species were, on average,
25 times higher than the differences within species. We
identified only one misidentified species out of 239
specimens in a genetic resource bank, so confirming the
accuracy of species identification in the banking system.
We also identified two potential composite species,
calling for further investigation using more samples.
The finding of large COI sequence differences between
species confirms the effectiveness of COI barcodes for
identifying Korean bird species. To bring greater
reliability to the identification of species, increased intra-
and interspecies sampling, as well as supplementation
of the mitochondrial barcodes with nuclear ones, is
needed.
ISSN
1016-8478 (print)
0219-1032 (online)
http://molcells.inforang.com/about/journal_view.html?uid=349
Language
English
URI
https://hdl.handle.net/10371/6485
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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