Browse

Identification of a Peptide Enhancing Mucosal and Systemic Immune Responses against EGFP after Oral Administration in Mice

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
Kim, Sae-Hae; Lee, Kyung-Yeol; Kim, Ju; Park, Seung-Moon; Park, BongKyun; Jang, Yong-Suk
Issue Date
2006
Publisher
한국분자·세포생물학회 = Korean Society of Molecular and Cellular Biology
Citation
Mol. Cells. 21, 244-250
Keywords
AdjuvantGangliosideMucosal ImmunizationPhage Display Library
Abstract
Gangliosides are receptors for various peptides and
proteins including neuropeptides, β-amyloid proteins,
and prions. Recently, the role of gangliosides in mucosal
immunization has attracted attention due to the
emerging interest in oral vaccination. Ganglioside
GM1 exists in abundance on the surface of the M cells
of Peyers patch, a well-known mucosal immunity induction
site. In the present study we identified a peptide
ligand for GM1 and tested whether it played a
role in immune induction. GM1-binding peptides were
selected from a phage-displayed dodecapeptide library
and one peptide motif, GWKERLSSWNRF, was fused
to the C-terminus of enhanced green fluorescent protein
(EGFP). The fusion protein, but not EGFP fused
with a control peptide, was concentrated around
Peyers patch after incubation in the lumen of the intestine
ex vivo. Furthermore, oral feeding of the fusion
protein but not control EGFP induced mucosal and
systemic immune responses against EGFP resembling
Th2-type immune responses.
ISSN
1016-8478 (print)
0219-1032 (online)
Language
English
URI
http://molcells.inforang.com/about/journal_view.html?uid=405

https://hdl.handle.net/10371/6488
Files in This Item:
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse