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Genetic characterization of canine rotavirus isolated from a puppy in korea and experimental reproduction of disease
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Bo Kyu | - |
dc.contributor.author | Song, Dae Sub | - |
dc.contributor.author | Jung, Kwon Il | - |
dc.contributor.author | Lee, Chul Seung | - |
dc.contributor.author | Park, Sung Jun | - |
dc.contributor.author | Oh, Jin Sik | - |
dc.contributor.author | An, Dong Jun | - |
dc.contributor.author | Yang, Jeong Sun | - |
dc.contributor.author | Moon, Hyoung Joon | - |
dc.contributor.author | Lee, Sang Sun | - |
dc.contributor.author | Yoon, Young Dhuk | - |
dc.contributor.author | Park, BongKyun | - |
dc.date.accessioned | 2009-08-07T03:27:49Z | - |
dc.date.available | 2009-08-07T03:27:49Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | J Vet Diagn Invest 19:78-83 | en |
dc.identifier.issn | 1040-6387 | - |
dc.identifier.uri | http://jvdi.org/cgi/content/abstract/19/1/78 | - |
dc.identifier.uri | https://hdl.handle.net/10371/6490 | - |
dc.description.abstract | Canine rotavirus was isolated from feces of a Korean Jindo dog with mild diarrhea, and the isolate was genetically characterized. Rotaviral antigen was detected in the feces using a commercial rotavirus antigen detection kit and cytopathic effects were observed in a cell line inoculated with the feces. The virus isolate (GC/KS05) was identified as subtype G3P[3] using reverse transcription polymerase chain reaction (RT-PCR). The strain displayed 98% and 90% identity with the VP7 genes of a canine rotavirus isolate (RV52/96) from Italy and the simian rotavirus strain (RRV) respectively. However, the GC/KS05 isolate exhibited only 83% and 82% identity, respectively, with the G3 serotype canine strains, RV198/95 and K9. Phylogenetic analysis of the VP7 and VP4 genes of GC/KS05 strain led to the classification of VP7 in a different cluster than other canine rotavirus VP7 genes, and VP4 within the cluster of canine rotavirus VP4 genes. The Korean isolate was thus more closely related to the RV52/96 isolate than the other isolates for which sequence data is available. Detailed analysis of the VP7 region revealed 6 amino acid variations between the new isolate and RV52/96. After 5 passages in cell culture, the GC/KS05 strain remained pathogenic for young pups, in which inoculation resulted in diarrhea and virus shedding in the feces. | en |
dc.language.iso | en | - |
dc.publisher | American Association of Veterinary Laboratory Diagnosticians (AAVLD) | en |
dc.subject | Canine rotavirus | en |
dc.subject | genotype G3P[3] | en |
dc.title | Genetic characterization of canine rotavirus isolated from a puppy in korea and experimental reproduction of disease | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 강보규 | - |
dc.contributor.AlternativeAuthor | 송대섭 | - |
dc.contributor.AlternativeAuthor | 정권일 | - |
dc.contributor.AlternativeAuthor | 이철승 | - |
dc.contributor.AlternativeAuthor | 박성준 | - |
dc.contributor.AlternativeAuthor | 오진식 | - |
dc.contributor.AlternativeAuthor | 안동준 | - |
dc.contributor.AlternativeAuthor | 양정선 | - |
dc.contributor.AlternativeAuthor | 문형준 | - |
dc.contributor.AlternativeAuthor | 이상선 | - |
dc.contributor.AlternativeAuthor | 윤용덕 | - |
dc.contributor.AlternativeAuthor | 박봉균 | - |
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