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Induction of protective immune responses against the challenge of Actinobacillus pleuropneumoniae by the oral administration of transgenic tobacco plant expressing ApxIIA toxin from the bacteria

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dc.contributor.authorLee, Kyung-Yeol-
dc.contributor.authorKim, Dong-Heon-
dc.contributor.authorKang, Tae-Jin-
dc.contributor.authorKim, Ju-
dc.contributor.authorChung, Gook-Hyun-
dc.contributor.authorYoo, Han Sang-
dc.contributor.authorArntzen, Charles J.-
dc.contributor.authorYang, Moon-Sik-
dc.contributor.authorJang, Yong-Suk-
dc.date.accessioned2009-08-07T06:40:18Z-
dc.date.available2009-08-07T06:40:18Z-
dc.date.issued2006-10-20-
dc.identifier.citationFEMS Immunol Med Microbiol 48: 381-389en
dc.identifier.issn0928-8244-
dc.identifier.urihttps://hdl.handle.net/10371/6513-
dc.description.abstractActinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia. Among the virulence factors, ApxIIA, a bacterial exotoxin, is reportedly expressed in many serotypes and is considered as a candidate for the development of a vaccine against the bacterial infection. Previously, we isolated a field strain of A. pleuropneumoniae serotype 2 in Korea and characterized its exotoxins to develop an oral vaccine. In this study, we initially confirmed the immunogenicity of ApxIIA expressed in Escherichia coli. We then developed transgenic tobacco expressing ApxIIA and tested its efficacy to induce a protective immune response against A. pleuropneumoniae infection after oral administration of the plant powder. We observed that protective immune responses were induced in mice after oral administration of the plant powder once a week for 4 weeks. Immunoassays revealed that the levels of antigen-specific immunoglobulin G against ApxIIA increased in mice that were fed a powder made from the transgenic plant, but not in mice fed a powder made from wild-type tobacco. Additionally, mice fed the transgenic plant powder were protected from an injection of a lethal dose of A. pleuropneumoniae. These results support that the transgenic plant may be a suitable candidate for an oral vaccine that could be used effectively against A. pleuropneumoniae infection.en
dc.description.sponsorshipThis work was supported by a grant from the Biogreen
21 program from RDA. Dr J. Kim was supported by
the Chonbuk National University postdoctoral training
program.
en
dc.language.isoen-
dc.publisherBlackwell Publishingen
dc.publisherFederation of European Microbiological Societies (FEMS)-
dc.subjectIedible vaccineen
dc.subjecttransgenic planten
dc.subjectActinobacillus pleuropneumoniaeen
dc.titleInduction of protective immune responses against the challenge of Actinobacillus pleuropneumoniae by the oral administration of transgenic tobacco plant expressing ApxIIA toxin from the bacteriaen
dc.typeArticleen
dc.contributor.AlternativeAuthor이경열-
dc.contributor.AlternativeAuthor김동헌-
dc.contributor.AlternativeAuthor강태진-
dc.contributor.AlternativeAuthor김주-
dc.contributor.AlternativeAuthor정국현-
dc.contributor.AlternativeAuthor유한상-
dc.contributor.AlternativeAuthor양문식-
dc.contributor.AlternativeAuthor장용석-
dc.identifier.doi10.1111/j.1574-695X.2006.00158.x-
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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