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A Study on the Property of LDH Isoenzymes of Rabbits
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chung, Duk-Jai | - |
dc.contributor.author | Kimm, Sung-Wun | - |
dc.date.accessioned | 2009-08-07T11:55:34Z | - |
dc.date.available | 2009-08-07T11:55:34Z | - |
dc.date.issued | 1971-12 | - |
dc.identifier.citation | Seoul J Med, Vol.12 No.4, pp. 209-216 | - |
dc.identifier.issn | 0582-6802 | - |
dc.identifier.uri | https://hdl.handle.net/10371/6592 | - |
dc.description.abstract | The present paper reported on the nature of
pyruvate inhibition of LDH isoenzymes with and without urea. The crude LDH isoenzymes were prepared from rabbit heart muscle for the H-LDH and from rabbit psoas muscle for the M~LDH, with the following conclusions. 1. It is possible to obtain 4~5 fold purification of the LDH isoenzymes from rabbit heart and psoas muscles only with ammonium sulfate fractionation and DEAE-cellu1eJse treatment. 2. In electrophoresis a new method of staining is proposed as follows; after electrophoresis with agarose gel a cellulose acetate strip, presoaked in the staining mixture, is overlapped on top of the agarose gel, followed by incubation and densitometry. In so doing, a kinetic study on the LDH isoenzymes could be possible through formazan staining, instead of UV spectrophotometry. 3. The H-LDH isoenzyme activity is more markedly inhibited than the M-LDH isoenzyme by pyruvate. 4. Under the presence of urea, pyruvate inhibition of H-LDH isoenzyme decreases, while that of M-LDH isoenzyme increases. | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 의과대학 | - |
dc.title | A Study on the Property of LDH Isoenzymes of Rabbits | - |
dc.type | SNU Journal | - |
dc.contributor.AlternativeAuthor | 장덕재 | - |
dc.contributor.AlternativeAuthor | 김승원 | - |
dc.citation.journaltitle | 서울 의대 잡지 | - |
dc.citation.journaltitle | 서울 의대 학술지 | - |
dc.citation.journaltitle | Seoul Journal of Medicine | - |
dc.citation.endpage | 216 | - |
dc.citation.number | 4 | - |
dc.citation.pages | 209-216 | - |
dc.citation.startpage | 209 | - |
dc.citation.volume | 12 | - |
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