S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 15 No.3 (1974)
토끼 대퇴근육에서 부분정제한 M4-LDH의 특성에 관한 연구
A Study on the Properties of M4-LDH Purified Partially from the Thigh Muscle of Rabbits
- 최영; 김승원
- Issue Date
- 서울대학교 의과대학
- Seoul J Med, Vol.15 No.3, pp. 153-162
- 1. The M.-LDH was partially purified approximately
14 fold of its activity from the thigh muscle
of rabbits by means of salting-out with ammonium
sulfate and of DEAE-cellulose column chromatography.
2. The activity of the M.-LDH was inhibited by
0.02M pyruvate to 22% of the original activity.
3. Sulfide ions. when added to the reacion system
using lactate as the substrate. accentuated the inhibition
already brought by pyruvate alone.
4. Cysteine. on the other hand. when treated
similarly as sulfide ions. decreased the magnitude
of pyruvate inhibition in its lower range of concentration.
but showed its own inhibitory effect
without regard to the pyruvate inhibition in its
higher range of concentration.
5. When lactate was used as substrate. both
sulfide ions and cysteine played as a non-competitive
6. When pyruvate was used as substrate. cysteine
showed an apparently allosteric effect. while sulfide
ions showed no particular effect different from
pyruvate induction itself.