S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 15 No.3 (1974)
각종동물조직 및 암조직의 LDH isoenzyme 및 RNase, RNase inhibitor 활성에 관한 연구
Studies on LDH Isoenzymes, RNase and RNA Inhibitor Activities in Varying Animals Including Parasites. and Cancer tissues
- 이기영; 박상철
- Issue Date
- 서울대학교 의과대학
- Seoul J Med, Vol.15 No.3, pp. 224-234
- The LDH isoenzyme and RNase activities of hepatic
'Of hepatopancreatic and muscle tissues from varying
species of animals were studied. The animals examined
are 1 species of echinodermate. 7 species of
mollusc. 2 species of arthropod. 1 species of chordate
and 6 species of vertebrate.
The LDH isoenzymes and RNase activities were
also examined on the parasites such as ascaris and
liver fluke along with their host tissues with reference
to host-parasite relationship. and the same
approach was conducted with stomach and cervical
cancers, comparying to their control tissues.
The following results were obtained.
1) The total activities of LDH from the hepatopancreatic
and muscle tissues of molluscs were
extremely low and the electrophoretic patterns of
LDH isoenzymes were quite different from those of
the equivalent tissues of mammals. The electrophoretic
separation of LDH isoenzymes in fishes.
was not clear with heavy tailing. as compared to
that of mammalian tissues. The specific activities
of tissue LDH of animals. seem to increase as animal
evolves. The specific activities of muscle LDH were
generally higher than those of hepatic or hepatopancreatic
2) The RNase activity patterns of hepatopancreas
and muscle of molluscs were quite different from
those of equivalent tissues of mammals.
It is noteworthy that the total RNase activities
turned out to be much less than the free RNase
activities in sea cucumber and most of molluscs.
unlike higher animals. by using SH reagent(pHMB)
which is well known to suppress the RNase inhibitor.
Thus. SH reagent supposedly inhibited RNase activity
or activated RNase inhibitor activity in lower
animals in contrast with higher animals. The total
RNase activities were generally higher in hepatopancreas
or liver than in muscle. contrary to LDH
3) The LDH isoenzymes in bovine liver fluke were electrophoretically separated into clear-cut 5 bands.
in contrast with lower animals. evidencing a close
host-parasite relationship. The specific activity of
LDH in liver fluke. however. was very low. as other
lower animals. The LDH isoenzymes in muscle of
swine ascaris was clearly fractionated into 3 bands
on elecrtophoresis, unlike lower animals.
The total RNase activity of liver fluke was
much lower than that of host liver. but the RNase
inhibitor activity was similar to that of host tissue.
and the activities of total RNase and RNase inhibitor
in muscle of ascaris were low. It is of
interest that SH reagent was proved to suppress
the RNase inhibitor. contrary to lower animals.
4) The total activities of LDH and RNase inhibitor
activities were higher in stomach and cervical
cancer tissues than in their normal control tissues.
and the electrophoretic patterns of LDH isoenzymes
of these cancer tissues were slightly different from
those of the control ones. The LDH-1 fraction was
prominent in stomach cancer tissue. and the activity
of LDH-4 fraction in cervical cancer tissue was
higher than that of control group.