마우스거식세포의 Mycobacterium fortuitum에 대한 반응양상

Abstract

Numerous studies on the interactions between microorganisms and phagocytes have suggested that the phagocytosis may be influenced by the nature of microorganisms, the metabolic states of the phagocytes and the physico-chemical nature of environment. etc. Especially, it has been well known that the attachment phase of phagocytosis may be enhanced by the immune sera containing specific antibodies and complements. But information is incomplete or missing on the mode of action of normal homologous and heterologous serum mixed in the medium of in vitro experiments on phagocytosis. Therefore, an experiment was performed to understand the influences of normal heterologous serum on the interaction between mouse macrophages and Mycobacterium /ortuitum which are pathogenic to mice. in the medium with bovine serum as heterologous serum and as a control in the medium without serum components. Thus, normal mouse macrophages were collected from the peritoneal cavity, pooled, suspended in the cell-maintenance medium, and mixed with supension of Mycobacterium fortuitum both in the medium with serum and in the medium without serum. The bacteria-macrophages suspensions in the Leighton tubes with cover-slip were incubated at 37'C, for 120 minutes, and the ratio of macrophages containing AFB to the total macrophages observed in the stained coverslip and viable bacterial counts in the supernatants of the medium in this experimental condition were checked at zero time(as a control), 30 min .. 60 min., 90 min., and 120 min. during incubation. The results obtained in this experiment were summarized as follows; 1. The rate of phagocytosis was more heightened in the medium with serum than that in the medium without serum, i.e, on phagocytosis of mouse macrophages, 2. Until 60 minutes of incubation the macrophagos in the medium with serum phagocytosed Mycobacterium fortuitum twice more rapidly than those in the medium without serum, but no significant difference in the rate of phagocytosis were found at 120 minutes. 3. The phagocytic index at 120 minutes in the medium with serum amounted to 0.4698 when calculated from the viable bacterial counts checked, and half-life of the viable bacterial counts in the supernatant was 71.1· minutes. 4. After 90 minutes of incubation, more than 5096 of macrophages in the stained cover-slip from the medium with serum contained AFB in the cytoplasm.