급성 일산화탄소 중독시 흰쥐 심근에 있어서 조직화학적 변화연구

Abstract

Carbon monoxide poisoning has been one of the seriM ous health problems last 20 years. The high incidence of accidents is mainly due to the public uses of anthracite coal briquettes as main domestic fuel for heating and cooking. Authour designed this study to elucidate the effects of the acute carbon monoxide poisoning on the myocardial tissue by histochemical approach and some of 105 rats were exposed to 1%, 0.7% and 0.4%CO gas mixture. The half lethal times were 30 min., 60min., and 120min. respectively. The control rats and lethal rats among 3 experimental groups were dissected immediately and the enzyme activities of succinic dehydrogenase (SDH), diphosphopyridine nucleotide reduced form (DPNH) and lactic dehydrogenase (LDH) were observed by histochemical staining method. Hematoxylin and eosin staining was made simultaneously and serum LDH. carboxyhemoglobin, hemoglobin, hematocrit and red cell count were measured. The findings obtained are as follows; 1. The average survival time of the I%CO, 0.796 CO and 0.4% CO exposure groups were 25min., 53 min. and 135min respectively. 2. The histological sections from rat after 30 min., 50 min. and 120 min. acute poisoning with 1%. 0.7% and 0.4% carbon monoxide revealed mild to moderate congestion and focal extravasation of erythrocytes in the cardiac muscles without necrotic changes. 3. The values of hemoglobin. hematocrit and red cell count showed marked increase in the experimental groups compared to the control group, but the difference among the experimental groups were found insignificant statistically. 4. The activity levels of SDH and DPNH were redused proportionally to the CO concentration and the exposure time. The activity level of LDH was slightly increased. These changes are statistically significant. The serum level of LDH decreased in the 120min. exposed group, but was not changed in the other exposure groups.