S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 18 No.4 (1977)
급성 일산화탄소 중독시 흰쥐 심근에 있어서 조직화학적 변화연구
A Histochemical Study ou the Myocardium of the Rat iu Acute Carbon Monoxide Poisoning
- 이영; 이영균; 윤덕로
- Issue Date
- 서울대학교 의과대학
- Seoul J Med, Vol.18 No.4, pp. 205-212
- Carbon monoxide poisoning has been one of the seriM
ous health problems last 20 years. The high incidence of accidents is mainly due to the public uses of
anthracite coal briquettes as main domestic fuel for
heating and cooking.
Authour designed this study to elucidate the effects
of the acute carbon monoxide poisoning on the
myocardial tissue by histochemical approach and some
of 105 rats were exposed to 1%, 0.7% and 0.4%CO
gas mixture. The half lethal times were 30 min.,
60min., and 120min. respectively.
The control rats and lethal rats among 3 experimental
groups were dissected immediately and the
enzyme activities of succinic dehydrogenase (SDH),
diphosphopyridine nucleotide reduced form (DPNH)
and lactic dehydrogenase (LDH) were observed by
histochemical staining method. Hematoxylin and eosin
staining was made simultaneously and serum LDH.
carboxyhemoglobin, hemoglobin, hematocrit and red
cell count were measured.
The findings obtained are as follows;
1. The average survival time of the I%CO, 0.796
CO and 0.4% CO exposure groups were 25min., 53
min. and 135min respectively.
2. The histological sections from rat after 30 min.,
50 min. and 120 min. acute poisoning with 1%. 0.7%
and 0.4% carbon monoxide revealed mild to moderate
congestion and focal extravasation of erythrocytes
in the cardiac muscles without necrotic changes.
3. The values of hemoglobin. hematocrit and red
cell count showed marked increase in the experimental
groups compared to the control group, but the
difference among the experimental groups were found
4. The activity levels of SDH and DPNH were redused
proportionally to the CO concentration and the
exposure time. The activity level of LDH was slightly
increased. These changes are statistically significant.
The serum level of LDH decreased in the 120min.
exposed group, but was not changed in the other