S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 21 No.1 (1980)
급, 만성 일산화탄소 중독이 이신 백서 자궁에 미치는 영향에 관한 연구 -병리조직학적 및 조직화학적 소견을 중심으로-
The Histo-pathological and Histo-chemical Studies on the Effects of Acute and Chronic Carbon Monoxide Poisoning in Gestated Rat Uterus
- 김인달; 윤덕로; 김용익
- Issue Date
- 서울대학교 의과대학
- Seoul J Med, Vol.21 No.1, pp. 55-68
- Carbon monoxide poisoning is very common home
accident and one of the main health hazards in
Korea, since Koreans use anthracite coal briquette as
domestic fuel. Among the many harmful effects of
CO poisoning, it is noteworthy that it increases the
pregnancy wastage rate and, in milder cases, impedes
the fetal development. But, in spite of the importance
of the CO poisoning in pregnancy, there have been very few reports on the histo-pathological changes.
which happen in the uterus of pregnant woman.
This study was designed to investigate the histo-pathological
and histo-chemical changes of CO poisoning
in gestated uterus. Gestated rats exposed to carbon
monoxide gas of 4, 500~5, OOOppm for 20 minutes at
the 16th Gestation day comprise acute CO poisoning
group. Chronic poisoning group were exposed to CO·
gas of 400ppm for 8 hours from 13th to 16th day of
the Gestation, therefore the total duration of exposure
was 32 hours. Among twenty three rats in each
group, eight were sacrificed at the end of the exposure
to observe the changes at the moment. The rest
fifteen were observed at the 20th gestation day that
is just before the spontaneous delivery. And, as control
groups, eight gestated rats were observed at the
16th day and thirty at the 20th day of the gestation.
The plain H&E staining and also histo-chemical'
staining were made to observe the extracted rat uteri.
Investigated enzymes were NADH diaphorase, SDH,_
LIm, alkaline phosphatase, and acid phosphatase.
Following results were observed:
1. In acute poisoning group sacrificed at the 16th
day, fibrinoid necrosis and infiltration of lymphatic
cells were observed in subplacental myometrium, and
the congestion was found in the inner myometrium.
In chronic exposure group, the common finding wasmild
congestion. In acute exposure group observed
at the 20th day, myometrium showed mild congestion
and lymphatic infiltration, but in chronic exposure
group, there was no difference from normal control
group of the same date.
2. The activity of NADH diaphorase was reduced
in both acute and chronic groups at the 16th day.
It regained the normal value at the 20th day.
3. The activity of SDH was reduced in both acute
and chronic exposure groups at the 16th day, but it
returned to the level of normal control group at the
4. The activity of LDH was reduced in acute ex'
posure group at the 16th day, but increased in
chronic exposure group of the same date. At the 20th
day, both showed no difference from normal control
5. The activity of alkaline phosphatase was redu-ced in both groups at the 16th day. At the 20th day
there was no difference between poisoning groups
.and control group.
6. The activity of acid phosphatase was low at the
16th day, but it showed almost same level with normal
control group at 20th day.