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Ca2+-activated K+ currents of pancreatic duct cells in guinea-pig
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Han-Wook | - |
dc.contributor.author | Li, Jingchao | - |
dc.contributor.author | Koo, Na-Youn | - |
dc.contributor.author | Piao, Zheng Gen | - |
dc.contributor.author | Hwang, Sung Min | - |
dc.contributor.author | Han, Jae-Woong | - |
dc.contributor.author | Lee, Jong-Heun | - |
dc.contributor.author | Choi, Han-Saem | - |
dc.contributor.author | Kim, Joong Soo | - |
dc.contributor.author | Park, Kyungpyo | - |
dc.date.accessioned | 2010-05-17T10:23:13Z | - |
dc.date.available | 2010-05-17T10:23:13Z | - |
dc.date.issued | 2004-12 | - |
dc.identifier.citation | Korean J Physiol Pharmacol 8: 335-338 | en |
dc.identifier.issn | 1226-4512 | - |
dc.identifier.uri | https://hdl.handle.net/10371/66604 | - |
dc.description.abstract | There are numerous studies on transepithelial transports in duct cells including Cl- and/or HCO3-. However, studies on transepithelial K+ transport of normal duct cells in exocrine glands are scarce. In the present study, we examined the characteristics of K+ currents in single duct cells isolated from guinea pig pancreas, using a whole-cell patch clamp technique. Both Cl- and K+ conductance were found with KCl rich pipette solutions. When the bath solution was changed to low Cl-, reversal potentials shifted to the negative side, -75±4 mV, suggesting that this current is dominantly selective to K+. We then characterized this outward rectifying K+ current and examined its Ca22+ dependency. The K+ currents were activated by intracellular Ca22+. 100 nM or 500 nM Ca22+ in pipette significantly (P<0.05) increased outward currents (currents were normalized, 76.8?7.9 pA, n=4 or 107.9?35.5 pA, n=6) at +100 mV membrane potential, compared to those with 0 nM Ca2+ in pipette (27.8?3.7 pA, n=6). We next examined whether this K+ current, recorded with 100 nM Ca2+ in pipette, was inhibited by various inhibitors, including Ba2+, TEA and iberiotoxin. The currents were inhibited by 40.4±% (n=3), 87.0±% (n=5) and 82.5±% (n=9) by 1 mM Ba2+, 5 mM TEA and 100 nM iberiotoxin, respectively. Particularly, an almost complete inhibition of the current by 100 nM iberiotoxin further confirmed that this current was activated by intracellular Ca2+. The K+ current may play a role in secretory process, since recycling of K+ is critical for the initiation and sustaining of Cl- or HCO32+ secretion in these cells. | en |
dc.description.sponsorship | This work was supported by a grant of the Korea Health
21 R&D project, Ministry of Health & Welfare, Republic of Korea (Grant No 01-PJ5-PG1-01CH12-0002). | en |
dc.language.iso | en | en |
dc.publisher | 대한생리약리학회 | en |
dc.subject | Duct cells | en |
dc.subject | Guinea pig | en |
dc.subject | Pancreas | en |
dc.subject | K+ currents | en |
dc.subject | Ca2+ | en |
dc.title | Ca2+-activated K+ currents of pancreatic duct cells in guinea-pig | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이한욱 | - |
dc.contributor.AlternativeAuthor | 구나윤 | - |
dc.contributor.AlternativeAuthor | 황성민 | - |
dc.contributor.AlternativeAuthor | 한재웅 | - |
dc.contributor.AlternativeAuthor | 이종훈 | - |
dc.contributor.AlternativeAuthor | 최한샘 | - |
dc.contributor.AlternativeAuthor | 김중수 | - |
dc.contributor.AlternativeAuthor | 박경표 | - |
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