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CXCR-4 Knockdown by small interfering RNA inhibits cell proliferation and invasion of oral squamous cell carcinoma cells.

DC Field Value Language
dc.contributor.authorHong, Ji-Soo-
dc.contributor.authorPai, Hyun-Kyung-
dc.contributor.authorHong, Kyoung-Ok-
dc.contributor.authorKim, Mi-Ae-
dc.contributor.authorKim, Ji-Hong-
dc.contributor.authorLee, Jae-Il-
dc.contributor.authorHong, Sam-Pyo-
dc.contributor.authorHong, Seong-Doo-
dc.date.accessioned2010-05-17T10:48:58Z-
dc.date.available2010-05-17T10:48:58Z-
dc.date.issued2009-02-
dc.identifier.citationJ Oral Pathol Med 2009; 38: 214–219en
dc.identifier.issn0904-2512-
dc.identifier.urihttps://hdl.handle.net/10371/66612-
dc.description.abstractBackground: Oral squamous cell carcinomas (OSCCs) are characterized by a high degree of local invasion and a high rate of metastases to cervical lymph nodes. Downregulation of CXCR-4 by siRNA inhibits invasion and growth of breast and colon cancer cells. However, there have been no reports on the downregulation of CXCR-4 by small interfering RNA (siRNA) in oral cancer cells.

Methods: We generated two stable CXCR-4-knockdown clones (KBsi and KOSCC-25Bsi) from the KB and KOSCC-25B OSCC cell lines by lentiviral delivery. In vitro invasion and cell proliferation assays were used to investigate the effect of CXCR-4 downregulation on cell proliferation and invasiveness in KBsi and KOSCC-25Bsi. Immunohistochemistry was performed to evaluate the correlation between CXCR-4 expression and proliferation in 26 OSCC tissue samples.

Results: CXCR4-knockdown OSCC cells showed reduced invasiveness. The invasiveness of KBsi decreased to 29.5% of the vector-infected controls, and KOSCC-25Bsi decreased to 38.1% of the control vector-infected cells (P < 0.05). The CXCR4-knockdown OSCC cells grew significantly slower than the vector-infected control cells. KBsi and KOSCC-25Bsi cells proliferated at 69.5% and 71.7%, respectively, of the rate of control vector-infected cells (P < 0.05). CXCR-4-positive group had significantly higher PCNA labeling index than CXCR-4-negative group in OSCC tissue samples.

Conclusion: These results suggest that the downregulation of CXCR-4 induces anti-proliferative and anti-invasive effects in OSCC and that CXCR-4 might be a useful target molecule for the treatment of OSCC.
en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.subjectCXCR-4 knockdownen
dc.subjectinvasionen
dc.subjectoral squamous cell carcinomaen
dc.subjectproliferationen
dc.subjectsiRNAen
dc.titleCXCR-4 Knockdown by small interfering RNA inhibits cell proliferation and invasion of oral squamous cell carcinoma cells.en
dc.typeArticleen
dc.contributor.AlternativeAuthor홍지수-
dc.contributor.AlternativeAuthor배현경-
dc.contributor.AlternativeAuthor홍경옥-
dc.contributor.AlternativeAuthor김미애-
dc.contributor.AlternativeAuthor이재일-
dc.contributor.AlternativeAuthor홍삼표-
dc.contributor.AlternativeAuthor홍성두-
dc.identifier.doi10.1111/j.1600-0714.2008.00671.x-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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