Ehrlich 복수암세포 Guanine Deaminase의 특성에 관한 연구

Abstract

Crude preparation of the guanine deaminase(EC 3.5.4.3.) was obtained from the Ehrlich ascites tumor cells, by means of salting-out with ammonium sulfate, and the properties of the enzyme were observed with the following conclusions. 1. The Km value of the crude GDA was o. 12mM. 2. 2M guanidine-HCl inhibited 75% of the activity of GDA, and all of the activity was lost after 2 hour incubation of the enzyme at 50"C. 3. The GDA showed a single broad spectrum of its pH versus activity profile ranging from pH 7.0 to 9.0. 4. There was no evidence of the presence of the natural inhibitor of the Ehrlich ascites tumor cell GDA in the mitochondrial fraction of the cell.