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In Vitro Bromodeoxyuridine Labeling Method of Human Carcinomas under Various Conditions
인체 암종에서의 in vitro Bromodeoxyuridine 표지 조건의 비교

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Authors
Kim, Woo Ho; Kim, Yong Il; Kim, Chul Woo; Chang, Mee Soo
Issue Date
1991-03
Publisher
Seoul National University College of Medicine
Citation
Seoul J Med, Vol.32 No.1, pp. 1-7
Keywords
Cell kineticsProliferation markerBromodeoxyuridineIn vitro labelingHuman carcinomasGastrointestinal carcinomas
Abstract
The proliferative activity of tumor cells in human gastric and colonic adenocarcinoma
was measured by means of in vitro incorporation of the thymidine analogue,
5-bromo-2'-deoxyuridine (BrdU), into the newly-synthesized DNA of fresh tumors. The
incorporation was optimized by incubation for 2 hours at 3-4 atm. of mixed gas (95%
02 + 5% C02) with 100 flM BrdU admixed in the culture media. Fresh specimens
up to 2 hours after resection retained their ability to incorporation, but their S-phase
fraction gradually decreased thereafter. The corporation rate was preserved better by
refrigeration than by storing at room temperature. With the advantage of its safety
and reliability in evaluation of the proliferative activity of human malignancy, in vitro
BrdU labeling technique could be recommended as a routine procedure to assess the
prognosis of the patient.
ISSN
0582-6802
Language
English
URI
https://hdl.handle.net/10371/6705
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 32 No.1 (1991)
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