An Experimental Study on the Cytolytic Effects of Natural Killer cells on Various Types of Lung Carcinoma Cell Lines with Augmented HLA Expressions by Gamma Interferon

Abstract

Lung carcinoma cell lines (NCI-N417, NCI-NI46, NCI-H69, PC-14) were evaluated on their expression of HLA-class I with W6/32 antibody and HLA-class II with L243 by flow cytometry. Also examined were the killing and/or cytolytic effects of allogeneic NK cells and T lymphocytes as effector cells to the lung carcinoma cell lines by chromium release assay, before and after treatments with gamma interferon. HLA class I expressions significantly increased after treatment with gamma interferon (P <0.05), whereas HLA class II expressions showed no differences (P > 0.05). The killing and/or cytolytic effects of allogeneic NK cells very significantly decreased after treatment with gamma interferon (P <0.001), while those of the T lymphocytes showed either no change or a tendancy to decrease (P > 0.05).