S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Enzymatically labeled chromosomal probes for in situ identification of human cells in xenogeneic transplant models
- Cho, Jae-jin; Malhi, Harmeet; Wang, Richard; Joseph, Brigid; Ludlow, John W.
- Issue Date
- Nature Publishing Group
- Nature Medicine 8, 1033-1036
- Analysis of the viability, differentiation, clonogenicity and function
of human stem/progenitor cells requires suitable xenograft
models. However, the identification of transplanted cells has
been generally difficult. Fluorescence in situ hybridization is a tedious
method for analyzing tissues, and localization of transplanted
cells with X or Y chromosome probes is limited by the
sparse signals produced. Therefore, we examined the possibility
of generating either pan-nuclear signals with a total human
DNA probe or multiple nuclear signals with a pan-centromeric
human DNA probe. The probes were labeled with digoxigenin
to make reaction products visible by light microscopy and to
allow the use of immunohistochemistry methods incorporating
various color schemes to demonstrate specific properties of
transplanted cells. The ability to localize all types of nucleated
human cells with such probes will facilitate studies of stem cell
biology and cell and gene therapy, as well as the development
of new animal models.
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