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Use of long-term cultured embryoid bodies may enhance cardiomyocyte differentiation by BMP2
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Yoon Young | - |
dc.contributor.author | Ku, Seung-Yup | - |
dc.contributor.author | Jang, Jiho | - |
dc.contributor.author | Oh, Sun Kyung | - |
dc.contributor.author | Kim, Hee Sun | - |
dc.contributor.author | Kim, Seok Hyun | - |
dc.contributor.author | Choi, Young Min | - |
dc.contributor.author | Moon, Shin Yong | - |
dc.date.accessioned | 2010-06-04T03:26:05Z | - |
dc.date.available | 2010-06-04T03:26:05Z | - |
dc.date.issued | 2008-10-31 | - |
dc.identifier.citation | Yonsei Med J. 2008;49(5):819-27 | en |
dc.identifier.issn | 0513-5796 (Print) | - |
dc.identifier.uri | https://hdl.handle.net/10371/67306 | - |
dc.description.abstract | PURPOSE: Human embryonic stem cells (hESCs) can proliferate for a prolonged period and differentiate into cardiomyocytes in vitro. Recent studies used bone morphogenetic protein 2 (BMP2) to generate cardiomyocytes from hESCs, however, all those studies used early embryoid bodies (EBs) and did not retrieve cardiomyocytes with a high yield. In this study, we treated long-term cultured EBs with BMP2 in order to promote differentiation into cardiomyocytes from hESCs. MATERIALS AND METHODS: hESC lines, including SNUhES3 and SNUhES4, were used in this study. Undifferentiated hESC colonies were detached to form EBs and cultured for up to 30 days. These long-term cultured EBs were differentiated into cardiomyocytes in serum-containing media. In our protocol, BMP2 was applied for 5 days after attachment of EBs. Cardiac specific markers, beating of differentiated cells and electron microscopic (EM) ultrastructures were evaluated and analyzed. RESULTS: Compared to 10-day or 20-day EBs, 30-day EBs showed a higher expression level of cardiac specific markers, Nkx2.5 and a-myosin heavy chain (aMHC). Treatment of BMP2 increased expression of cardiac troponin (cTn) I and a-actinin when evaluated at 20 days after attachment of 30-day EBs. Beating of differentiated cells was observed from 7 to 20 days after attachment. Moreover, EM findings demonstrated fine structures such as Z bands in these differentiated cardiomyocytes. These long-term cultured EBs yielded cardiomyocytes with an efficiency of as high as 73.6% when assessed by FACS. CONCLUSION: We demonstrated that the use of long-term cultured EBs may enhance differentiation into cardiomyocytes from hESCs when treated with BMP2. | en |
dc.language.iso | en | en |
dc.publisher | Yonsei University College of Medicine | en |
dc.subject | Bone Morphogenetic Protein 2/*pharmacology | en |
dc.subject | Cell Culture Techniques | en |
dc.subject | Cell Line | en |
dc.subject | Cell Proliferation | en |
dc.subject | Embryonic Stem Cells/cytology/*drug effects | en |
dc.subject | Humans | en |
dc.subject | Myocytes, Cardiac/*cytology | en |
dc.subject | Pluripotent Stem Cells/cytology/drug effects | en |
dc.subject | Signal Transduction | en |
dc.subject | Cell Differentiation | - |
dc.title | Use of long-term cultured embryoid bodies may enhance cardiomyocyte differentiation by BMP2 | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김윤영 | - |
dc.contributor.AlternativeAuthor | 구승엽 | - |
dc.contributor.AlternativeAuthor | 장지호 | - |
dc.contributor.AlternativeAuthor | 오선경 | - |
dc.contributor.AlternativeAuthor | 김희선 | - |
dc.contributor.AlternativeAuthor | 김석현 | - |
dc.contributor.AlternativeAuthor | 최영민 | - |
dc.contributor.AlternativeAuthor | 문신용 | - |
dc.identifier.doi | 10.3349/ymj.2008.49.5.819 | - |
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